Considerations for RT-qPCR in molecular point of care testing devices

Considerations for RT-qPCR in molecular point of care testing devices

The COVID-19 pandemic has accelerated the uptake of point of care testing (POCT), with simple, user-administered tests becoming commonplace in schools, places of work and people’s homes. Antigen POCT has been pivotal in SARS-CoV-2 detection, particularly where reverse transcription PCR (RT-PCR) cannot be delivered in a timely manner. However, the use of antigen tests isn’t always appropriate due to variable sensitivity. RT-PCR – or RT-qPCR if using real-time detection – offers greater sensitivity and specificity. Leveraging this technology in POCT brings lab quality results to the point of need, providing rapid, accurate and actionable results to clinicians.

Read more

The BiosearchTech Blog

Most popular

Explore the vast applications of KASP genotyping for plant breeding programmes

Posted on Tue, Sep 20, 2022 @ 04:45 AM

Modern agriculture faces unprecedented challenges: increasing global temperatures, water scarcity, less arable land to ...
Read more

Topics: Agrigenomics, PCR-based genotyping

Where are all the therapeutic aptamers?

Posted on Tue, Aug 09, 2022 @ 12:10 PM

New approaches that use aptamers as targeting agents may open the door to wider clinical use in the future
Read more

Topics: Oligo synthesis and modifications, Nucleic acid therapeutics

Choosing the best qPCR probe chemistry for your assay

Posted on Thu, Jul 28, 2022 @ 08:15 AM

Let the application be your guide when deciding between LNA, MGB and BHQplus  The many different chemistries available ...
Read more

Topics: Molecular Diagnostics, Oligo synthesis and modifications, qPCR

Pivoting to monkeypox diagnostics

Posted on Thu, Jul 07, 2022 @ 03:38 PM

Since 13 May 2022, reports of monkeypox cases began to appear globally in regions not endemic to the monkeypox virus.1 ...
Read more

Topics: Molecular Diagnostics, Disease detection

Genetic solutions to infectious diseases in aquaculture

Posted on Thu, Jun 30, 2022 @ 02:12 PM

This is the second blog in a two-part series exploring solutions for infectious diseases in aquaculture. In the first ...
Read more

Topics: Agrigenomics, PCR-based genotyping, NGS-based genotyping

Make your diagnostic workflows more adaptable

Posted on Mon, Jun 06, 2022 @ 12:47 PM

Since early 2020, diagnostic laboratories have had to manage patient sample quantities being in a state of flux. Demand ...
Read more

Topics: SARS-CoV-2, Nucleic acid extraction, Disease detection

Increasing resistance to aquaculture disease

Posted on Tue, Apr 19, 2022 @ 09:00 AM

This is the first blog in a two-part series exploring solutions for infectious diseases in aquaculture. This blog post ...
Read more

Topics: Agrigenomics, Genomics

Pandemic reflections: Adapting to a constant flux in testing priorities

Posted on Tue, Apr 05, 2022 @ 02:55 PM

What’s changed for clinical labs as we enter the third year of COVID-19 testing? COVID-19, the illness caused by ...
Read more

Topics: SARS-CoV-2

Optimising automated high-throughput pathogen detection workflows

Posted on Wed, Mar 30, 2022 @ 09:00 AM

While both centralised and decentralised molecular diagnostics (MDx) testing models play an important role in patient ...
Read more

Topics: Molecular Diagnostics, qPCR

Overcoming manufacturing challenges associated with nucleic acid therapeutics

Posted on Thu, Mar 24, 2022 @ 04:15 PM

This is the second blog in a two-part series exploring how oligonucleotide modifications can support the advancement of ...
Read more

Topics: Oligo synthesis and modifications, Nucleic acid therapeutics

Improving the sustainability of oil palm breeding programmes using marker-assisted selection

Posted on Tue, Mar 08, 2022 @ 01:00 PM

African oil palm (Elaeis guineensis Jacq.) is a highly productive perennial crop that is used widely in food, ...
Read more

Topics: Agrigenomics, qPCR, PCR-based genotyping

Considerations for RT-qPCR in molecular point of care testing devices

Posted on Tue, Mar 01, 2022 @ 01:11 PM

The COVID-19 pandemic has accelerated the uptake of point of care testing (POCT), with simple, user-administered tests ...
Read more

Topics: Molecular Diagnostics, qPCR

Nucleic acid therapeutics for next-generation disease management

Posted on Fri, Feb 25, 2022 @ 12:47 PM

This is the first blog post in a two-part series exploring how oligonucleotide modifications can support the ...
Read more

Topics: Oligo synthesis and modifications, Nucleic acid therapeutics

Society can’t handle the caveats to each type of COVID-19 test

Posted on Fri, Feb 11, 2022 @ 02:58 PM

But measurement science could help steer us in a better, more informed direction COVID-19 tests can indicate the ...
Read more

Topics: qPCR, SARS-CoV-2

Leveraging quenching efficiency of Black Hole Quencher dyes for PCR and beyond

Posted on Tue, Jan 25, 2022 @ 12:30 PM

More innovative applications for Black Hole Quencher (BHQ) dyes In imaging and detection assay development, innovative ...
Read more

Topics: Oligo synthesis and modifications

Increasing hybrid rice breeding efficiency with high-throughput genotyping

Posted on Tue, Jan 18, 2022 @ 01:03 PM

Rice is the primary staple for more than half the world's population.1 With so many people relying on the crop for ...
Read more

Topics: Agrigenomics, qPCR, PCR-based genotyping

Pandemic reflections: in the face of SARS-CoV-2 variants, COVID-19 testing remains essential

Posted on Tue, Jan 11, 2022 @ 10:00 AM

What’s changed for clinical labs as we enter the third year of COVID-19 testing? COVID-19, the illness caused by ...
Read more

Topics: SARS-CoV-2

Pandemic reflections: unprecedented demand drives COVID-19 test developers to get creative

Posted on Tue, Dec 28, 2021 @ 07:30 AM

What’s changed for clinical labs as we enter the third year of COVID-19 testing? COVID-19, the illness caused by ...
Read more

Topics: SARS-CoV-2

Technical bulletin: Omicron impact on SARS-CoV-2 mutation analysis and investigation

Posted on Thu, Dec 23, 2021 @ 09:45 AM

The emergence of the Omicron lineage has quickly escalated to be a variant of concern by the World Health Organization ...
Read more

Topics: Molecular Diagnostics, SARS-CoV-2

Pandemic reflections: the world is short staffed

Posted on Tue, Dec 21, 2021 @ 04:52 PM

What’s changed for clinical labs as we enter the third year of COVID-19 testing?
Read more

Topics: SARS-CoV-2

Oligonucleotide synthesis basics: Key takeaways for post-synthesis processing

Posted on Thu, Dec 16, 2021 @ 02:39 PM

This is the final blog post in a three-part educational series about best practices for in-house oligonucleotide ...
Read more

Topics: Oligo synthesis and modifications

Ensuring our diagnostic assays detect the Omicron variant

Posted on Thu, Dec 09, 2021 @ 02:24 PM

On 22 November 2021, The Network for Genomics Surveillance in South Africa detected a new SARS-CoV-2 variant, named the ...
Read more

Topics: Molecular Diagnostics, SARS-CoV-2

A targeted GBS technology for all breeding communities

Posted on Tue, Dec 07, 2021 @ 02:11 PM

The decisions made by molecular breeding programmes have a substantial impact on the world around us, from productivity ...
Read more

Topics: Agrigenomics, Next Generation Sequencing (NGS), NGS-based genotyping

Extensions to the IVDR - what diagnostics manufacturers and suppliers need to know

Posted on Thu, Dec 02, 2021 @ 03:25 PM

We're excited to announce that the proposal to extend certain key provisions of the In Vitro Diagnostic Regulation ...
Read more

Topics: Molecular Diagnostics

Streamlining diagnostic workflows for common respiratory diseases, such as SARS-CoV-2, influenza and RSV

Posted on Tue, Nov 30, 2021 @ 02:29 PM

A fourth wave of COVID-19 has already emerged in many countries and a newly classified variant of concern, Omicron ...
Read more

Topics: Molecular Diagnostics, SARS-CoV-2, Disease detection

Tackling breeding challenges for polyploid crops

Posted on Tue, Nov 23, 2021 @ 07:00 AM

Many cultivated crops with agronomic significance, including wheat, peanut, oat, banana, potato, coffee and strawberry, ...
Read more

Topics: Agrigenomics, NGS-based genotyping

Innovative applications for Black Hole Quencher dyes

Posted on Tue, Nov 16, 2021 @ 02:14 PM

This is the first blog post in a two-part series highlighting how Black Hole Quencher™ dyes can be incorporated into ...
Read more

Topics: Molecular Diagnostics, Oligo synthesis and modifications

Oligonucleotide synthesis basics: 4 key takeaways for in-house oligo production

Posted on Tue, Nov 02, 2021 @ 09:41 AM

This is the second blog post in a three-part educational series about best practices for in-house oligonucleotide ...
Read more

Topics: Oligo synthesis and modifications

Technical bulletin: SARS-CoV-2 mutation analysis and investigation

Posted on Thu, Oct 14, 2021 @ 03:01 PM

SARS-CoV-2 mutations and associated new variants are being reported regularly in the media and scientific literature. ...
Read more

Topics: Molecular Diagnostics, SARS-CoV-2

8 qPCR amplification plot questions answered

Posted on Thu, Sep 30, 2021 @ 08:00 AM

When results from your qPCR assays aren’t quite what you expected, it can be challenging to understand what went wrong. ...
Read more

Topics: qPCR

Can COVID-19 testing be made more sustainable during prolonged uncertainty?

Posted on Mon, Sep 27, 2021 @ 12:36 PM

In September 2021, Delta persists as the most prevalent variant worldwide1 due to its high infectivity, causing surges ...
Read more

Topics: Molecular Diagnostics, qPCR, SARS-CoV-2

The surprising global impacts of Europe's In Vitro Diagnostic Regulation (IVDR)

Posted on Thu, Sep 16, 2021 @ 10:15 AM

A shortcut to understanding how to prepare for IVDR In today’s global environment, even small regulatory changes in one ...
Read more

Topics: Molecular Diagnostics

Oligonucleotide synthesis basics: Introduction to phosphoramidite chemistry

Posted on Tue, Sep 14, 2021 @ 12:00 PM

This is the first blog post in a three-part educational series about best practices for in-house oligonucleotide ...
Read more

Topics: Oligo synthesis and modifications

4 steps to optimise sample collection for aquaculture genotyping

Posted on Tue, Sep 07, 2021 @ 12:59 PM

Aquaculture breeding programmes adopt genomic technologies, including genotyping, to increase genetic gain for specific ...
Read more

Topics: Agrigenomics, PCR-based genotyping, Nucleic acid extraction

Tips for early-stage MDx companies launching new tests during COVID-19 times

Posted on Thu, Sep 02, 2021 @ 12:00 PM

The COVID-19 pandemic brought tremendous change to the molecular diagnostics (MDx) industry, delivering opportunities ...
Read more

Topics: Molecular Diagnostics, SARS-CoV-2

Wastewater surveillance: a critical tool in pandemic management

Posted on Tue, Aug 31, 2021 @ 07:48 AM

As SARS-CoV-2 variants continue to threaten public health, officials are looking beyond clinical tests to track ...
Read more

Topics: SARS-CoV-2

Technical bulletin: Emerging SARS-CoV-2 mutations and their effect on the immune response

Posted on Wed, Aug 18, 2021 @ 01:45 PM

Emerging SARS-CoV-2 variants of concern and variants of interest are defined by agencies such as the World Health ...
Read more

Topics: SARS-CoV-2

The importance of sample processing for successful plant genotyping by NGS

Posted on Thu, Aug 12, 2021 @ 04:51 PM

Informed plant breeding decisions help drive the field of agrigenomics towards ever-increasing agricultural ...
Read more

Topics: Agrigenomics, Next Generation Sequencing (NGS), Nucleic acid extraction

Don't underestimate the complexity of your sample composition

Posted on Wed, Aug 04, 2021 @ 02:34 PM

A common mistake when initiating an agrigenomics project is failing to consider specific sample composition before ...
Read more

Topics: Agrigenomics, Nucleic acid extraction

Advancing aquaculture breeding programmes with genomic tools

Posted on Tue, Jun 15, 2021 @ 09:15 AM

The global demand for fish and fish products continues to increase as consumers focus on health-conscious ways to add ...
Read more

Topics: Agrigenomics, Genomics

Genomic surveillance: 3 techniques to detect and characterise SARS-CoV-2 variants

Posted on Wed, May 19, 2021 @ 09:34 AM

As the global population gains greater access to vaccines for COVID-19, scientists and health officials continue to ...
Read more

Topics: Molecular Diagnostics, Genomics, SARS-CoV-2

How do SARS-CoV-2 variants affect PCR assays for COVID-19 testing?

Posted on Tue, May 04, 2021 @ 09:03 AM

Researchers have identified several variants of SARS-CoV-2 since they sequenced the original viral strain responsible ...
Read more

Topics: Molecular Diagnostics, SARS-CoV-2

Best practices for implementing targeted genotyping by sequencing

Posted on Wed, Dec 02, 2020 @ 08:53 AM

In breeding programmes, selections based on a single or small number of markers to target traits in marker assisted ...
Read more

Topics: Agrigenomics, Genomics, NGS-based genotyping

Building resilience into SARS-CoV-2 testing workflows: considerations for choosing an RNA isolation method

Posted on Wed, Oct 21, 2020 @ 09:00 AM

Clinical and diagnostic labs worldwide are still experiencing supply chain challenges with their SARS-CoV-2 testing ...
Read more

Topics: Molecular Diagnostics, Genomics, SARS-CoV-2

How high-throughput genotyping automation and KASP marker libraries connect research labs with commercial breeders to accelerate crop improvement

Posted on Wed, Oct 07, 2020 @ 09:31 AM

“In July of 2015, I was a member of the radish genome project team. My task was to examine genetic diversity of radish ...
Read more

Topics: Genomics, PCR-based genotyping

Employee spotlight: Meet Darshna "Dusty" Vyas

Posted on Mon, Sep 14, 2020 @ 08:15 AM

Darshna "Dusty" Vyas has been a Senior Scientist at LGC specialising in plant genetics for the past 8 years Her ...
Read more

Topics: Agrigenomics, Genomics, PCR-based genotyping

Pressing COVID-19 testing issues of today and the near future

Posted on Wed, Aug 26, 2020 @ 07:57 AM

Following up with the panelists from our recent COVID-19 webinar, Part 3 of 3 In the previous two blog posts in this ...
Read more

Topics: Molecular Diagnostics, Genomics, SARS-CoV-2

Sensitivity and accuracy in COVID-19 testing

Posted on Wed, Aug 19, 2020 @ 07:56 AM

Following up with the panelists from our recent COVID-19 webinar, Part 2 of 3 In the face of a rapidly evolving ...
Read more

Topics: Molecular Diagnostics, Genomics, SARS-CoV-2

Scaling up COVID-19 viral testing

Posted on Wed, Aug 12, 2020 @ 07:47 AM

Following up with the panelists from our recent COVID-19 webinar, Part 1 of 3 The COVID-19 pandemic has presented ...
Read more

Topics: Molecular Diagnostics, Genomics, SARS-CoV-2

Enabling a rapid response to COVID-19 by Honeywell and LGC, Biosearch Technologies

Posted on Wed, Jul 29, 2020 @ 12:40 PM

As countries worldwide begin to ease lockdown restrictions during the pandemic and prepare to return to “normal” ...
Read more

Topics: Molecular Diagnostics, Genomics, qPCR, SARS-CoV-2

Establishment of KASP marker library for genetic diversity analysis in Brassica rapa  crops

Posted on Tue, Jul 14, 2020 @ 08:17 AM

Brassica  rapa crops are important sources of food, feed, and fuel in many parts of the world and provide a unique ...
Read more

Topics: Agrigenomics, Genomics, PCR-based genotyping

Robust supply chains and open testing systems: How our AgBio roots meet the FDA's EUA process to enable solutions to the COVID-19 pandemic

Posted on Tue, May 19, 2020 @ 08:18 AM

It may seem strange that a company known for transforming the global seed breeding industry is uniquely positioned to ...
Read more

Topics: Molecular Diagnostics, Genomics, qPCR, SARS-CoV-2

Behind the scenes: How our oligo synthesis team contributes to the reliability of your assays

Posted on Thu, Apr 30, 2020 @ 02:13 PM

We’re not rejecting you, just your oligo PCR is arguably one of the most enabling technologies of modern molecular ...
Read more

Topics: Molecular Diagnostics, Oligo synthesis and modifications, Genomics, SARS-CoV-2

Expanded SeqSNP support with new NGS lab in Middleton, WI

Posted on Tue, Apr 14, 2020 @ 09:15 AM

LGC, Biosearch Technologies is excited to announce the expansion of SeqSNP targeted genotyping by sequencing (GBS) ...
Read more

Topics: Agrigenomics, Genomics, NGS-based genotyping

When you don’t need an EUA for SARS-CoV-2 testing

Posted on Fri, Apr 10, 2020 @ 03:00 PM

On March 25, 2020, the US Food and Drug Administration (FDA) held the first of a series of virtual town halls they will ...
Read more

Topics: Molecular Diagnostics, SARS-CoV-2

New NGS service lab now open in Middleton, WI for expanded global support

Posted on Wed, Apr 01, 2020 @ 09:27 AM

Back in September 2018, LGC acquired Lucigen Corporation. The ISO accredited site is based in Middleton, Wisconsin and ...
Read more

Topics: Genomics, Next Generation Sequencing (NGS), NGS-based genotyping

Addressing COVID-19 together: LGC, Biosearch Technologies and Shanghai Liferiver Biotechnology Co., Ltd.

Posted on Tue, Mar 31, 2020 @ 10:13 AM

In the nearly three months since the first confirmed case of COVID-19, the outbreak has become a global crisis, with ...
Read more

Topics: Molecular Diagnostics, Oligo synthesis and modifications, Genomics, SARS-CoV-2

What diagnostic test makers and CLIA labs in the US need to know about Emergency Use Authorization (EUA)

Posted on Tue, Mar 24, 2020 @ 11:58 AM

*Since the original publication of this blog, LGC, Biosearch Technologies is now the only acceptable commercial ...
Read more

Topics: Molecular Diagnostics, Genomics, SARS-CoV-2

Coronavirus outbreak: Collaboration to meet the challenge

Posted on Mon, Mar 16, 2020 @ 12:30 PM

Coronavirus, or COVID-19, is an infectious virus that is spreading rapidly around the world and the World Health ...
Read more

Topics: Molecular Diagnostics, Genomics, SARS-CoV-2

Molecular diagnostics: how can NGS live up to its promises?

Posted on Wed, Jan 29, 2020 @ 09:37 AM

The development of new genomic sequencing techniques and bioinformatics analysis in the last decade has led to massive ...
Read more

Topics: Molecular Diagnostics, Genomics, PCR-based genotyping, Next Generation Sequencing (NGS)

Back to basics - the crucial step of sample collection

Posted on Wed, Jan 15, 2020 @ 09:37 AM

Best practice in sample collection for SeqSNP targeted genotyping by sequencing projects When good goes in, great comes ...
Read more

Topics: Genomics, PCR-based genotyping, NGS-based genotyping

Genomic selection: can it accelerate livestock breeding?

Posted on Wed, May 01, 2019 @ 08:44 AM

Livestock supports the livelihoods and food security of almost 1.3 billion people worldwide, contributing 40% of the ...
Read more

Topics: Agrigenomics, Genomics, Next Generation Sequencing (NGS), NGS-based genotyping

The women of LGC, Biosearch Technologies

Posted on Fri, Mar 29, 2019 @ 09:24 AM

 We've heard it said for years, "TV will rot your brain". But in the case of our three colleagues below, that ...
Read more

Topics: Genomics

Agrigenomics: how can NGS contribute to global food security?

Posted on Wed, Mar 06, 2019 @ 08:33 AM

“Experience of artificial fertilisation, such as is effected with ornamental plants in order to obtain new variations ...
Read more

Topics: Agrigenomics, Genomics, PCR-based genotyping, Next Generation Sequencing (NGS), NGS-based genotyping

A way to prevent type 1 diabetes?

Posted on Wed, Feb 13, 2019 @ 08:08 AM

Blindness, cardiovascular disease, and stroke are all long-term health problems that can be caused by type 1 diabetes ...
Read more

Topics: Genomics, PCR-based genotyping, Disease detection

How targeted GBS-driven genomic selection can help you get to market faster

Posted on Wed, Jan 16, 2019 @ 11:22 AM

Plant and animal breeding programmes often take generations to improve quality and yield but using a genotyping ...
Read more

Topics: Agrigenomics, Genomics, Next Generation Sequencing (NGS), NGS-based genotyping

Genomic selection: methods in crop and animal breeding

Posted on Wed, Jan 09, 2019 @ 08:48 AM

Genomic selection: 6 factors to consider when choosing between targeted GBS and microarrays
Read more

Topics: Genomics, NGS-based genotyping

Our hungry planet: new tools in agrigenomics are key to food security

Posted on Tue, Dec 11, 2018 @ 08:32 AM

Food security is a major global threat and traditional methods of plant and animal breeding will not be sufficient to ...
Read more

Topics: Agrigenomics, Next Generation Sequencing (NGS), NGS-based genotyping

Accelerating rice improvement in South Asia

Posted on Thu, Jul 26, 2018 @ 09:26 AM

Diversity is the spice of life and is also key to breeding rice that delivers increased yields. Rice is a crucial ...
Read more

Topics: PCR-based genotyping

Revolutionising cancer treatment one Array at a time

Posted on Tue, Jul 10, 2018 @ 09:05 AM

While the science of pharmacogenomics has been around for years, its popularity is starting to pick up steam as ...
Read more

Topics: Genomics

Pack it up, pack it in, let me begin: A look at the future of DNA data storage

Posted on Thu, Jun 28, 2018 @ 09:13 AM

Imagine a time capsule the size of a shoebox that holds all the information currently on the internet that can be ...
Read more

Topics: Genomics, Nucleic acid extraction

Warning! Pitfalls ahead: How to avoid the most common Stellaris RNA FISH mistakes

Posted on Tue, May 29, 2018 @ 08:32 AM

Ever wake up in the morning just feeling like a rock star, or maybe like Francis Crick, Rosalind Franklin or Maurice ...
Read more

Topics: RNA FISH, Genomics

Shining a light on gene expression and regulation one cell at a time

Posted on Thu, Apr 19, 2018 @ 10:51 AM

To truly understand complex diseases such as cancer, scientists need access to tools that don’t just average data ...
Read more

Topics: RNA FISH, Genomics

The Women of LGC

Posted on Thu, Mar 29, 2018 @ 08:45 AM

Ask any little girl what they want to be when they grow up and you may hear things like, princess, ballerina, pop star, ...
Read more

Topics: Genomics

In case you missed it: Stellaris RNA FISH lunch-and-learn

Posted on Tue, Jan 16, 2018 @ 08:35 AM

Stellaris® RNA FISH (fluorescence in situ hybridization) is an RNA visualization method that allows simultaneous ...
Read more

Topics: RNA FISH, Genomics

Tackling food crisis through cost effective, target enriched sequencing

Posted on Wed, Dec 13, 2017 @ 08:07 AM

With the global population predicted to reach 9 billion people within the next 35 years, we will need to double the ...
Read more

Topics: Agrigenomics, Genomics, NGS-based genotyping

New SNPs identified in the hunt for new genetic weapons targeting leaf rust in wheat

Posted on Tue, Nov 21, 2017 @ 09:25 AM

Wheat is one of the three most important crops for human and livestock feed. With an ever-increasing global population, ...
Read more

Topics: Agrigenomics, Genomics, PCR-based genotyping

Super-crops helping to feed Africa

Posted on Fri, Nov 03, 2017 @ 09:08 AM

Our world population is growing at a tremendous rate and by 2050, Africa’s population alone is set to hit over 2.5 ...
Read more

Topics: Agrigenomics, Genomics, PCR-based genotyping

How genotyping is aiding in the fight against malaria

Posted on Wed, Sep 20, 2017 @ 08:11 AM

3.2 billion people across 106 countries and territories, live in areas at risk of malaria transmission. The serious and ...
Read more

Topics: Genomics, PCR-based genotyping, Disease detection

Lunch & Learn: Stellaris RNA FISH

Posted on Thu, Jul 06, 2017 @ 08:07 AM

Recently LGC Genomics hosted a lunch and learn Stellaris® seminar at the Helen Diller Cancer Institute at UCSF with our ...
Read more

Topics: RNA FISH, Genomics

It’s official! Personal Health becomes first China demonstration laboratory site for LGC’s Nexar System

Posted on Wed, May 03, 2017 @ 07:05 AM

LGC's Genomics Division has signed a memorandum of understanding (MOU) with Chinese health management expert Personal ...
Read more

Topics: Genomics, qPCR

Digital PCR – improving reproducible molecular methods in the clinic

Posted on Thu, Apr 06, 2017 @ 08:40 AM

A recent study coordinated by LGC and published in the journal Analytical Chemistry, has demonstrated the potential of ...
Read more

Topics: Real-time PCR

Shining a light on how Zika virus may spread from mothers to human fetuses

Posted on Mon, Mar 06, 2017 @ 08:37 AM

New research has, for the first time, found evidence suggesting the molecular epidemiology of how the Zika virus ...
Read more

Topics: RNA FISH, Disease detection

Water – Back to Basics for Thermal Cycling

Posted on Tue, Feb 28, 2017 @ 08:13 AM

It’s time to forget what you know about heating your PCR plates for end-point PCR. Water is the way to go. Where ...
Read more

Overcoming drought-intolerance in maize to feed the growing Asian population

Posted on Wed, Feb 15, 2017 @ 07:53 AM

  Demand for maize is increasing due to an increased number of people transitioning to meat-based diets driving demand ...
Read more

Topics: Genomics, PCR-based genotyping

Automate to Accelerate and Scale-up SNP-based Genotyping Workflows!

Posted on Thu, Feb 02, 2017 @ 07:14 AM

The advent of and ongoing improvements to next-generation sequencing technologies is driving the continuing and ...
Read more

Topics: Genomics

Multiple Misunderstandings: Learning from Genetic Determinism

Posted on Mon, Jan 23, 2017 @ 08:12 AM

Nature versus nurture. It’s a tale as old as time. When Craig Venter made the comment after the human genome sequence ...
Read more

Topics: Genomics

Helping to Feed the World with Genotyping by Sequencing

Posted on Tue, Jan 17, 2017 @ 07:42 PM

Many areas critical to improved agricultural production require robust and scalable genotyping platforms to enable the ...
Read more

Topics: Agrigenomics, Genomics, PCR-based genotyping, NGS-based genotyping

Faster, stronger, quicker: SSR to KASP will get you there

Posted on Mon, Jan 09, 2017 @ 07:05 AM

Screen more markers faster and bring crops with superior traits to the market quicker.
Read more

Topics: PCR-based genotyping

Multiplex your qPCR and Walk-away

Posted on Thu, Dec 15, 2016 @ 08:17 AM

It seems everyone wants to do more with less, and automate the process as well.  But this can cause a number of ...
Read more

Topics: Genomics, qPCR

A Stellar-is Year in the Making

Posted on Wed, Dec 07, 2016 @ 08:05 AM

 It wouldn't be fair to close out 2016 without showcasing the stellar year that Stellaris® RNA FISH has had. Take a ...
Read more

Topics: RNA FISH, Genomics

Molded for our Purpose

Posted on Mon, Dec 05, 2016 @ 07:30 AM

With the discovery of the double helix, we learned how genes are copied and how genetic information was passed on. From ...
Read more

Topics: Genomics

Buddy Up: The Double Helix

Posted on Mon, Nov 28, 2016 @ 07:15 AM

The secret of life. That’s what Francis Crick and James Watson claimed they had discovered back in Cambridge, England ...
Read more

Topics: Genomics

Genes, Proteins & DNA: Our Hereditary Software

Posted on Mon, Nov 21, 2016 @ 07:17 AM

In the early 1900s, scientists found that the function of genes was to make proteins, disproving the earlier idea that ...
Read more

Topics: Genomics

"X" Marks the Spot: A Closer Look at Chromosomes

Posted on Mon, Nov 14, 2016 @ 07:28 AM

The strands of DNA in your cells are organized into pieces called chromosomes. Chromosomes are further organized into ...
Read more

Topics: Genomics

Excel Autocorrect Fails the Genomics Community - Are your Results Excellent or Excel-not?

Posted on Thu, Sep 08, 2016 @ 09:30 AM

Researchers from the Baker IDI Heart and Diabetes Institute in Melbourne, Australia have blamed a default autocorrect ...
Read more

Topics: Genomics

qPCR-based Molecular Diagnostics at the Forefront of Zika Surveillance

Posted on Tue, Aug 23, 2016 @ 02:00 PM

As the Zika virus outbreak spreads, a wave of international concern has grown, and scientists have scrambled to ...
Read more

Topics: Real-time PCR

Know Your Oligo Mod: Thio C6 Linker

Posted on Thu, Jul 14, 2016 @ 02:00 PM

We continue our “Know Your Oligo Mod” series with another versatile custom oligonucleotide modification: the Thio C6 ...
Read more

Topics: Oligo synthesis and modifications

A Complete Ecosystem to Accelerate Breeding Programs [Infographic]

Posted on Tue, Jun 28, 2016 @ 02:00 PM

Modern breeding methods rely on the identification and utilization of molecular markers associated with traits ...
Read more

Topics: Agrigenomics

DNA Technology is now LGC Biosearch Technologies

Posted on Tue, Jun 14, 2016 @ 03:52 PM

For DNA Technology’s existing customers, don’t fret! The online ordering process will remain the same, as will the team ...
Read more

Photoblog: Behind the scenes of oligo synthesis

Posted on Wed, May 18, 2016 @ 03:00 PM

OLIGO SYNTHESIS PHOTO GLIMPSE We’ve recently captured our talented manufacturing teams hard at work building oligos. ...
Read more

Topics: Molecular Diagnostics, Real-time PCR, Oligo synthesis and modifications

Nova is the new black

Posted on Thu, Apr 14, 2016 @ 02:00 PM

LGC Biosearch Technologies has developed a new qPCR probe format powered by Black Hole Quencher® technology known as ...
Read more

Topics: Real-time PCR

Learn about the latest developments in technologies for molecular breeding programs

Posted on Thu, Mar 31, 2016 @ 02:00 PM

At this year’s 24th annual Plant & Animal Genome (PAG) conference, LGC Biosearch Technologies hosted a symposium ...
Read more

Imaging Stellaris Assays Part III: Creating Professional, Publication-ready Images

Posted on Thu, Mar 10, 2016 @ 02:00 PM

Here is the final installment of our “Imaging Stellaris Assays” blog series. In our two previous posts, we’ve gone over ...
Read more

Topics: RNA FISH

Introducing BHQnova Probes for qPCR - Join the Early Access Program

Posted on Mon, Feb 29, 2016 @ 02:00 PM

LGC Biosearch Technologies is announcing the upcoming release of a new qPCR probe format known as BHQnova™ probes. This ...
Read more

Topics: Real-time PCR

Designing Stellaris RNA FISH Probes Part I: Increasing Probe Count

Posted on Thu, Jan 14, 2016 @ 02:00 PM

Stellaris® RNA FISH is a novel technology that allows for the detection of single or clustered RNAs in fixed cells and ...
Read more

Topics: RNA FISH

Know Your Oligo Mod: 3' Spacer C3

Posted on Thu, Oct 15, 2015 @ 02:15 PM

This week, we continue our Know Your Oligo Mod series and we will focus on a very basic, yet extremely versatile ...
Read more

Topics: Real-time PCR, Oligo synthesis and modifications

Imaging Stellaris Assays Part II: How to Acquire Stunning Images

Posted on Thu, Sep 24, 2015 @ 02:30 PM

Here we continue our “Imaging Stellaris Assays” blog series.  We last left off talking about the most important ...
Read more

Topics: RNA FISH

Runaway RNA Stays Put

Posted on Thu, Sep 17, 2015 @ 02:00 PM

The cleavage and polyadenylation event, the final trimming in the molecular mRNA barbershop, normally releases both the ...
Read more

Topics: RNA FISH

Imaging Stellaris Assays Part I: Get to Know Your Microscope

Posted on Thu, Aug 27, 2015 @ 05:00 PM

Stellaris® FISH is an RNA detection technology performed by means of in situ hybridization. This technology and all of ...
Read more

Topics: RNA FISH

The AGCT’s of DNA Synthesis

Posted on Tue, Aug 11, 2015 @ 02:00 PM

Chemically synthesized DNA has become an indispensable tool for molecular biologists in the fields of medicine, ...
Read more

Topics: Oligo synthesis and modifications

Know Your Oligo Mod: BHQ® (Black Hole Quencher®) Dyes

Posted on Thu, Jul 23, 2015 @ 02:00 PM

In this installment, we will cover the basics of LGC Biosearch Technologies’ line of proprietary quencher chromophores ...
Read more

Topics: Oligo synthesis and modifications

And the Consensus (Sequence) is...

Posted on Thu, Jun 11, 2015 @ 02:31 PM

Studying the expression level of RNA transcripts is often complicated by the fact that many DNA sequences are ...
Read more

Topics: Real-time PCR, RNA FISH

Research Highlight: Bringing Vision Research into Focus

Posted on Thu, May 28, 2015 @ 02:14 PM

Gene expression and molecular biology research in vision and ophthalmology presents unique challenges to researchers ...
Read more

Topics: RNA FISH

MIQE Goes Digital with Open Access qPCR Guide

Posted on Thu, May 21, 2015 @ 02:00 PM

Since its inception, qPCR has been recognized as a powerful molecular method. However, the application of this ...
Read more

Topics: Real-time PCR

FISH-ing for Fusions: A unique approach to fusion gene mRNA & pre-mRNA detection in cancer

Posted on Fri, May 15, 2015 @ 01:00 PM

This past April, Biosearch attended the American Association of Cancer Research (AACR) conference in Philadelphia, PA ...
Read more

Topics: RNA FISH

How Much is Your qPCR Assay Really Costing You?

Posted on Thu, Apr 02, 2015 @ 02:00 PM

In the setup of a qPCR experiment, one of the first decisions to make is which detection method to use. This generally ...
Read more

Topics: Real-time PCR

Research Highlight: Localizing long non-coding RNA (lncRNA)

Posted on Thu, Mar 12, 2015 @ 02:00 PM

A recent paper published in Molecular Cell by Dimitrova et al. from the lab of Dr. Tyler Jacks at MIT, used Stellaris® ...
Read more

Topics: RNA FISH

The 4Cs of Stellaris® RNA FISH Buffers

Posted on Thu, Feb 26, 2015 @ 02:00 PM

Buffers are often overlooked and taken for granted until the day comes when a peculiar result is observed and its ...
Read more

Topics: RNA FISH

Know Your Oligo Mod: Phosphorothioate Bonds

Posted on Thu, Feb 12, 2015 @ 02:00 PM

This post in our continuing series, “Know your oligo mod” will cover phosphorothioate bonds, which are available for ...
Read more

Topics: Oligo synthesis and modifications

BHQplus Probes Optimize Multiplex Competitive qRT-PCR in Diagnostics

Posted on Thu, Jan 29, 2015 @ 02:00 PM

In a recent webinar, Dr. James Willey describes a two color fluorometric assay for more robust quantitation of nucleic ...
Read more

Topics: Real-time PCR

A Successful Duo for Accurate, Speedy, and Economical SNP Genotyping

Posted on Thu, Jan 08, 2015 @ 11:30 AM

Single Nucleotide Polymorphisms (SNPs) have been widely studied for over 15 years, and have applications in forensics, ...
Read more

Topics: Real-time PCR, PCR-based genotyping

Know Your Oligo Mod: Methylene Blue

Posted on Tue, Dec 09, 2014 @ 02:02 PM

The chemical synthesis of oligonucleotides allows for the incorporation of a wealth of modifications to an oligo ...
Read more

Topics: Oligo synthesis and modifications

Unraveling the Neural Web: Stellaris® RNA FISH for Neuroscience Research

Posted on Thu, Nov 13, 2014 @ 03:45 PM

Neuroscience gene expression research can come with many hurdles, especially when working with brain tissue or mixed ...
Read more

Topics: RNA FISH

Check out our new website qPCR Design Lab @ qPCRdesign.com

Posted on Thu, Oct 23, 2014 @ 02:00 PM

Biosearch Technologies has launched qPCRdesign.com, a new microsite targeted specifically for your quantitative PCR ...
Read more

Topics: Real-time PCR

Delivering Oligos by Nanomole Units

Posted on Wed, Oct 15, 2014 @ 11:00 AM

Different oligonucleotide manufacturers deliver probes and primers in a variety of different measured units including ...
Read more

Topics: Real-time PCR, Oligo synthesis and modifications

RT-qPCR Molecular Diagnostics Assist Workers at the Forefront of Ebola Outbreak.

Posted on Thu, Sep 25, 2014 @ 11:30 AM

The largest recorded Ebola Virus Disease outbreak in history continues to devastate the West African region. This ...
Read more

Topics: Molecular Diagnostics, Real-time PCR

One Step too Many? Simplify your qPCR Protocol.

Posted on Thu, Feb 27, 2014 @ 10:02 AM

Don’t waste time performing an extra step. Cut back on pipetting and order a ValuMix™ Assay to have your probes and ...
Read more

Topics: Real-time PCR

30 Years: The Impact of PCR

Posted on Thu, Sep 05, 2013 @ 09:29 AM

Do you remember when the most popular method of DNA amplification involved multiple hybridizations, followed by single ...
Read more

Topics: Real-time PCR

RT-qPCR and the Stellaris FISH Method

Posted on Fri, Apr 26, 2013 @ 10:32 AM

A "Referral from the Doctor" Blog Article- Quantitative reverse transcription polymerase chain reaction (RT-qPCR) is a ...
Read more

Topics: Real-time PCR, RNA FISH

Biosearch Technologies Responds To China Influenza H7N9 Outbreak By Providing H7N9 Detection Panel

Posted on Mon, Apr 15, 2013 @ 06:00 AM

Biosearch Technologies, Inc. (Biosearch) announced today that the company has implemented a rapid response to the ...
Read more

Stellaris® Foundation Genes: GAPDH

Posted on Thu, Apr 11, 2013 @ 09:30 AM

Stellaris probe sets targeting glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA were among the first designed at ...
Read more

Topics: RNA FISH

Biosearch Technologies, UMDNJ Stellaris® Probe Alliance - NextGen Gene Expression™, SNP FISH™, and iceFISH™ in Research and Clinical Applications

Posted on Mon, Mar 18, 2013 @ 08:43 AM

Biosearch Technologies, Inc. (Biosearch), a leading supplier of sophisticated oligonucleotide components to the rapidly ...
Read more

Topics: RNA FISH

Biosearch Technologies Announces Acquisition of European Companies DNA Technology and VitraBio

Posted on Thu, Feb 21, 2013 @ 06:00 AM

Biosearch Technologies, Inc. (Biosearch), a leading supplier of sophisticated, custom-manufactured oligonucleotides and ...
Read more

Biosearch Technologies Launches Catalogued Stellaris™ RNA FISH Assays

Posted on Mon, Jun 18, 2012 @ 08:08 AM

Biosearch Technologies, Inc. (Biosearch), a leading supplier of sophisticated oligonucleotide components to the rapidly ...
Read more

Topics: RNA FISH

Single molecule viral RNA analysis using Stellaris™ FISH technology

Posted on Wed, May 30, 2012 @ 01:00 PM

  Image courtesy of: http://www.cdc.gov/h1n1flu/images.htm A cooperative effort between researchers at Mount Sinai ...
Read more

Topics: RNA FISH

qPCR Probes at work: Innovative CLIA test for NSCLC staging

Posted on Tue, Mar 06, 2012 @ 11:00 AM

Non-squamous, Non-small cell lung cancer (NSCLC), the most common cause of death in the US and globally, has recently ...
Read more

Topics: Molecular Diagnostics, Real-time PCR

Factors affecting fluorophore performance in qPCR

Posted on Wed, Jan 11, 2012 @ 09:00 AM

A "Referral from the Doctor" Blog Article- In qPCR, fluorescent modified oligos may be either linear or non-linear in ...
Read more

Topics: Real-time PCR

The 'Monte Carlo' Effect in qPCR

Posted on Wed, Nov 09, 2011 @ 11:00 AM

A "Referral from the Doctor" Blog Article- The mathematical model of PCR is based on the assumption that each DNA ...
Read more

Blogging about Stellaris™ RNA FISH at Singlemoleculefish.com

Posted on Mon, Oct 24, 2011 @ 12:24 PM

Biosearch Technologies is starting a new sister blog at singlemoleculefish.com where all articles will focus on topics ...
Read more

Topics: RNA FISH

Fluorescence Quenching Mechanisms for Dual-Labeled Probes

Posted on Wed, Jun 29, 2011 @ 07:44 AM

A "Referral from the Doctor" Blog Article- Fluorescent probes are used in biochemical assays to monitor specific events ...
Read more

Topics: Real-time PCR

Multiplexing with Xanthenes

Posted on Wed, Jun 08, 2011 @ 09:13 AM

A "Referral from the Doctor" Blog Article- Quantitative real-time PCR (qPCR) was developed as a means to detect the ...
Read more

Topics: Real-time PCR

qPCR troubleshooting: interpreting amplification curves and diagnosing problems

Posted on Thu, May 05, 2011 @ 10:15 AM

If you run quantitative real-time PCR (qPCR) assays, the chances are that you’ve seen some strange looking ...
Read more

Topics: Real-time PCR

The Latest Scientific Streak in Fashion

Posted on Thu, Dec 02, 2010 @ 10:12 AM

I’ve always wondered why scientists have earned themselves this infamous reputation of dressing poorly and ...
Read more

Between ROX and a Hard Place

Posted on Wed, Nov 10, 2010 @ 09:12 AM

A "Referral from the Doctor" Blog Article- ROX in qPCR Quantitative real-time RT-PCR was developed as a means of ...
Read more

Topics: Real-time PCR

Biosearch Technologies and Seegene Finalize Oligonucleotide Supply and BHQ®, CAL Fluor® and Quasar® Licensing Agreements

Posted on Wed, Oct 20, 2010 @ 08:12 AM

Biosearch Technologies, Inc. (Biosearch), a leading supplier of sophisticated oligonucleotide components to the rapidly ...
Read more

Topics: Real-time PCR

Visit Biosearch at IVD Technology's Virtual Conference and Tradeshow

Posted on Thu, Sep 23, 2010 @ 02:32 PM

Biosearch Technologies is proud to sponsor the first IVD Technology Virtual Conference and Tradeshow. This free online ...
Read more

Topics: Molecular Diagnostics

Biosearch Technologies Expands Licensing of QIAGEN Scorpions® Patents

Posted on Thu, Aug 12, 2010 @ 08:41 AM

Biosearch Technologies today announces that it has entered into a new license relationship with QIAGEN (NASDAQ: QIA, ...
Read more

Topics: Real-time PCR

Taq facts

Posted on Tue, Aug 03, 2010 @ 08:14 AM

When the polymerase chain reaction (PCR) was first described, the Klenow fragment of Escherichia coli DNA Polymerase I ...
Read more

Topics: Real-time PCR, qPCR

PilotDx Oligos: En Route to IVD

Posted on Thu, Jul 22, 2010 @ 08:07 AM

It can be difficult to transition a product from research to commercialization. It’s simply not feasible to conduct ...
Read more

Topics: Molecular Diagnostics

Pipetting for qPCR

Posted on Wed, Jun 09, 2010 @ 08:41 AM

A "Referral from the Doctor" Blog Article- Research trends in laboratories today increasingly steer towards gene ...
Read more

Topics: Real-time PCR

Black Hole Scorpion® Primers - Killer probes for qPCR

Posted on Wed, Mar 24, 2010 @ 08:57 AM

A "Referral from the Doctor" Blog Article-   Scorpions®Primers are dual-labeled FRET probes that combine a Molecular ...
Read more

Topics: Real-time PCR

Molecular Beacons - Lights in the storm

Posted on Wed, Mar 10, 2010 @ 11:05 AM

A "Referral from the Doctor" Blog Article- Molecular Beacons are a special type of dual-labeled oligonucleotide probe. ...
Read more

Topics: Real-time PCR

Dual-labeled BHQ probes - Performance to “dye” for

Posted on Wed, Feb 24, 2010 @ 10:20 AM

A "Referral from the Doctor" Blog Article-   A BHQ® probe is a dual-labeled oligonucleotide covalently labeled with a ...
Read more

Topics: Real-time PCR

This Year's MVP (Most Valuable Practice): Touchdown PCR

Posted on Thu, Feb 04, 2010 @ 10:39 AM

A "Referral from the Doctor" Blog Article-   The aim of the Polymerase Chain Reaction (PCR) method is to amplify or ...
Read more

Topics: Real-time PCR

The Grand Ballet: Wine and Biotechnology

Posted on Wed, Jan 27, 2010 @ 11:15 AM

At the third qPCR Symposium, held November 9th -12th in Millbrae, CA, ETS laboratories (ETS) scientist Rich DeScenzo ...
Read more

Topics: Real-time PCR

An Alternative to DNAse I heat inactivation: LiCl precipitation

Posted on Wed, Jan 13, 2010 @ 10:04 AM

A "Referral from the Doctor" Blog Article- Genomic DNA contamination has been a hot topic for qPCR researchers since ...
Read more

Topics: Real-time PCR

A Quick Jump Start to Multiplexing qPCR

Posted on Wed, Jan 06, 2010 @ 10:01 AM

By now, you've no doubt read about the reductions in error, cost and time that can be achieved by multiplexing ...
Read more

Topics: Real-time PCR

Slope Efficiency Equals Manageable and Corrected PCR

Posted on Wed, Dec 09, 2009 @ 12:01 PM

A "Referral from the Doctor" Blog Article-   ε = m.c.2 (shall I explain? slope efficiency equals manageable and ...
Read more

Topics: Real-time PCR

Bringing Back a Champion: Competitive Quantitative Real-time RT-PCR

Posted on Wed, Nov 18, 2009 @ 08:08 AM

A "Referral from the Doctor" Blog Article- Competitive qRT-PCR has become a lost art. Early titans of probe-based PCR ...
Read more

Topics: Real-time PCR

The New Favorite Reference Gene: 36B4, a.k.a. RPLP0

Posted on Wed, Oct 14, 2009 @ 08:06 AM

A "Referral from the Doctor" Blog Article- Ribosomes are comprised of two subunits, one small (40S) and one large ...
Read more

Topics: Real-time PCR, Oligo synthesis and modifications

What Genes are Hot, What Genes are Not

Posted on Wed, Oct 07, 2009 @ 02:41 PM

 A "Referral from the Doctor" Blog Article-  Top 10 Reference Genes for probe-based Real-time PCR in 2009: • Acidic ...
Read more

Topics: Real-time PCR

Labeling Oligos with Internal BHQ Dyes

Posted on Tue, Sep 29, 2009 @ 08:16 AM

  When to use an internal Black Hole Quencher (BHQ) label in a fluorescence-quenched probe for real-time qPCR. When the ...
Read more

Topics: Real-time PCR, Oligo synthesis and modifications

Salt-Free Primer Pair Option Now Available

Posted on Tue, Sep 22, 2009 @ 08:08 AM

Biosearch Technologies will now be offering the option of salt-free primer pairs for purchase with any probe order. A ...
Read more

Topics: Real-time PCR, Oligo synthesis and modifications

Subscribe to our blog

About LGC, Biosearch Technologies

LGC, Biosearch Technologies is the complete Genomics portfolio from LGC. Providing genomic analysis tools, instrumentation and services to the genomic scientific discovery sector worldwide, with focus on across ag bio, pharma and molecular diagnostics. Visit our home page to view our products and services.

Posts by popularity

Follow @BiosearchTech on Twitter

Become a Fan on Facebook!