Time to read: 2 minute read
Updated : Thu, August 12, 2021 @ 11:51 PM
Originally published : Thu, Aug 12, 2021 @ 06:51 PM
Updated : Thu, August 12, 2021 @ 11:51 PM
Originally published : Thu, Aug 12, 2021 @ 06:51 PM
Informed plant breeding decisions help drive the field of agrigenomics towards ever-increasing agricultural productivity. But effective decisions cannot be made without strong underlying NGS data, which is dependent upon an extensive and complex workflow.
Sample processing is the first step in the next generation sequencing (NGS) workflow, and decisions made at this point impact whether low- or high-quality DNA/RNA is obtained, which directly affects the success of NGS. Given the importance of these initial decisions, they deserve significant consideration before embarking on any agrigenomics project. Read more to discover what factors should be considered before getting started.
We have all heard the phrase “good data in, good data out”, but how do you get high-quality input? It starts with good sample collection and extraction.
The following key considerations should be addressed before getting started:
The importance of good starting material cannot be overstated, and each sample has specific attributes that need to be identified and accounted for as you design your extraction and purification. Extracting DNA from plant cells can be more challenging than animal cell extraction given the toughness of plant cell walls, and starting material is often compromised in an effort to overcome this barrier.
Avoid common pitfalls to get your workflow off to the best start by considering:
Never underestimate the importance of quality sample collection and prep in your plant breeding workflow. Extra consideration during this phase will help remove challenges downstream, produce the highest quality data, and set the bar for how successful your NGS project can be.
Learn more about how LGC, Biosearch Technologies can help optimise sample preparation for NGS applications.
LGC, Biosearch Technologies is the complete Genomics portfolio from LGC. Providing genomic analysis tools, instrumentation and services to the genomic scientific discovery sector worldwide, with focus on across ag bio, pharma and molecular diagnostics. Visit our home page to view our products and services.
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