How to select an effective nucleic acid isolation method for human diagnostics

How to select an effective nucleic acid isolation method for human diagnostics

Molecular testing protocols for pathogen detection and diagnosing human diseases require reliable and high-quality isolation of genetic material from diverse sample types. There are many extraction and purification technologies available. Before deciding which isolation method best suits your application, read this blog that walks you through key considerations...

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A way to simplify design and interpretation of your qPCR assays

Posted on Fri, May 05, 2023 @ 09:34 AM

How does a BHQ dye contribute to producing superior data from your assays?
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Topics: Molecular diagnostics, Oligo synthesis and modifications, qPCR, Human healthcare, General science

Taq Polymerase: What is it and its applications

Posted on Fri, Aug 26, 2022 @ 07:58 AM

When the polymerase chain reaction (PCR) was first described, the Klenow fragment of Escherichia coli DNA Polymerase I ...
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Topics: qPCR, General science

Choosing the best qPCR probe chemistry for your assay

Posted on Thu, Jul 28, 2022 @ 10:15 AM

Let the application be your guide when deciding between LNA, MGB and BHQplus  The many different chemistries available ...
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Topics: Molecular diagnostics, Oligo synthesis and modifications, qPCR, Human healthcare, General science

Optimising automated high-throughput pathogen detection workflows

Posted on Wed, Mar 30, 2022 @ 11:00 AM

While both centralised and decentralised molecular diagnostics (MDx) testing models play an important role in patient ...
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Topics: Molecular diagnostics, qPCR, Human healthcare, General science

Improving the sustainability of oil palm breeding programmes using marker-assisted selection

Posted on Tue, Mar 08, 2022 @ 03:00 PM

African oil palm (Elaeis guineensis Jacq.) is a highly productive perennial crop that is used widely in food, ...
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Topics: Agrigenomics, qPCR, PCR-based genotyping, Human healthcare, Applied agrigenomics, General science

Considerations for RT-qPCR in molecular point of care testing devices

Posted on Tue, Mar 01, 2022 @ 03:11 PM

The COVID-19 pandemic has accelerated the uptake of point of care testing (POCT), with simple, user-administered tests ...
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Topics: Molecular diagnostics, qPCR, Human healthcare, General science

Society can’t handle the caveats to each type of COVID-19 test

Posted on Fri, Feb 11, 2022 @ 04:58 PM

But measurement science could help steer us in a better, more informed direction COVID-19 tests can indicate the ...
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Topics: qPCR, SARS-CoV-2, Human healthcare, General science

Increasing hybrid rice breeding efficiency with high-throughput genotyping

Posted on Tue, Jan 18, 2022 @ 03:03 PM

Rice is the primary staple for more than half the world's population.1 With so many people relying on the crop for ...
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Topics: Agrigenomics, qPCR, PCR-based genotyping

8 qPCR amplification plot questions answered

Posted on Thu, Sep 30, 2021 @ 10:00 AM

When results from your qPCR assays aren’t quite what you expected, it can be challenging to understand what went wrong. ...
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Topics: qPCR, Human healthcare, General science

Can COVID-19 testing be made more sustainable during prolonged uncertainty?

Posted on Mon, Sep 27, 2021 @ 02:36 PM

In September 2021, Delta persists as the most prevalent variant worldwide1 due to its high infectivity, causing surges ...
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Topics: Molecular diagnostics, qPCR, SARS-CoV-2, Human healthcare, General science

Enabling a rapid response to COVID-19 by Honeywell and LGC, Biosearch Technologies

Posted on Wed, Jul 29, 2020 @ 02:40 PM

As countries worldwide begin to ease lockdown restrictions during the pandemic and prepare to return to “normal” ...
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Topics: Molecular diagnostics, Genomics, qPCR, SARS-CoV-2

Robust supply chains and open testing systems: How our AgBio roots meet the FDA's EUA process to enable solutions to the COVID-19 pandemic

Posted on Tue, May 19, 2020 @ 10:18 AM

It may seem strange that a company known for transforming the global seed breeding industry is uniquely positioned to ...
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Topics: Molecular diagnostics, Genomics, qPCR, SARS-CoV-2

It’s official! Personal Health becomes first China demonstration laboratory site for LGC’s Nexar System

Posted on Wed, May 03, 2017 @ 09:05 AM

LGC's Genomics Division has signed a memorandum of understanding (MOU) with Chinese health management expert Personal ...
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Topics: Genomics, qPCR, Human healthcare, General science

Digital PCR – improving reproducible molecular methods in the clinic

Posted on Thu, Apr 06, 2017 @ 10:40 AM

A recent study coordinated by LGC and published in the journal Analytical Chemistry, has demonstrated the potential of ...
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Topics: qPCR, Human healthcare, General science

Multiplex your qPCR and Walk-away

Posted on Thu, Dec 15, 2016 @ 10:17 AM

It seems everyone wants to do more with less, and automate the process as well.  But this can cause a number of ...
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Topics: Genomics, qPCR, General science

qPCR-based molecular diagnostics at the forefront of Zika surveillance

Posted on Tue, Aug 23, 2016 @ 04:00 PM

As the Zika virus outbreak spreads, a wave of international concern has grown, and scientists have scrambled to ...
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Topics: qPCR, Human healthcare, General science

Photoblog: Behind the scenes of oligo synthesis

Posted on Wed, May 18, 2016 @ 05:00 PM

OLIGO SYNTHESIS PHOTO GLIMPSE We’ve recently captured our talented manufacturing teams hard at work building oligos. ...
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Topics: Molecular diagnostics, Oligo synthesis and modifications, qPCR, General science

Nova is the new black

Posted on Thu, Apr 14, 2016 @ 04:00 PM

LGC Biosearch Technologies has developed a new qPCR probe format powered by Black Hole Quencher™ technology known as ...
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Topics: qPCR, Human healthcare, General science

Know your oligo mod: 3' Spacer C3

Posted on Thu, Oct 15, 2015 @ 04:15 PM

This week, we continue our Know your oligo mod blog series and we will focus on a very basic, yet extremely versatile ...
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Topics: Oligo synthesis and modifications, qPCR, Human healthcare, General science

And the Consensus (Sequence) is...

Posted on Thu, Jun 11, 2015 @ 04:31 PM

Studying the expression level of RNA transcripts is often complicated by the fact that many DNA sequences are ...
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Topics: RNA FISH, qPCR, Human healthcare, General science

How Much is Your qPCR Assay Really Costing You?

Posted on Thu, Apr 02, 2015 @ 04:00 PM

In the setup of a qPCR experiment, one of the first decisions to make is which detection method to use. This generally ...
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Topics: qPCR, Human healthcare, General science

Optimising multiplex competitive qRT-PCR in diagnostics with BHQplus probes

Posted on Thu, Jan 29, 2015 @ 04:00 PM

In a webinar linked below, Dr. James Willey describes a two colour fluorometric assay for more robust quantitation of ...
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Topics: qPCR, Human healthcare, General science

A Successful Duo for Accurate, Speedy, and Economical SNP Genotyping

Posted on Thu, Jan 08, 2015 @ 01:30 PM

Single Nucleotide Polymorphisms (SNPs) have been widely studied for over 15 years, and have applications in forensics, ...
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Topics: qPCR, PCR-based genotyping, Applied agrigenomics, General science

Check out our new website qPCR Design Lab @ qPCRdesign.com

Posted on Thu, Oct 23, 2014 @ 04:00 PM

Biosearch Technologies has launched qPCRdesign.com, a microsite targeted specifically for your quantitative PCR needs. ...
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Topics: qPCR, Human healthcare, General science

Delivering Oligos by Nanomole Units

Posted on Wed, Oct 15, 2014 @ 01:00 PM

Different oligonucleotide manufacturers deliver probes and primers in a variety of different measured units including ...
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Topics: Oligo synthesis and modifications, qPCR, Human healthcare, General science

RT-qPCR Molecular Diagnostics Assist Workers at the Forefront of Ebola Outbreak.

Posted on Thu, Sep 25, 2014 @ 01:30 PM

The largest recorded Ebola Virus Disease outbreak in history continues to devastate the West African region. This ...
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Topics: Molecular diagnostics, qPCR, Human healthcare, General science

One Step too Many? Simplify your qPCR Protocol.

Posted on Thu, Feb 27, 2014 @ 12:02 PM

Don’t waste time performing an extra step. Cut back on pipetting and order a ValuMix™ Assay to have your probes and ...
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Topics: qPCR, Human healthcare, General science

30 Years: The Impact of PCR

Posted on Thu, Sep 05, 2013 @ 11:29 AM

Do you remember when the most popular method of DNA amplification involved multiple hybridizations, followed by single ...
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Topics: qPCR, Human healthcare, General science

RT-qPCR and the Stellaris FISH Method

Posted on Fri, Apr 26, 2013 @ 12:32 PM

A "Referral from the Doctor" Blog Article- Quantitative reverse transcription polymerase chain reaction (RT-qPCR) is a ...
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Topics: RNA FISH, qPCR, Human healthcare, General science

qPCR Probes at work: Innovative CLIA test for NSCLC staging

Posted on Tue, Mar 06, 2012 @ 01:00 PM

Non-squamous, Non-small cell lung cancer (NSCLC), the most common cause of death in the US and globally, has recently ...
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Topics: Molecular diagnostics, qPCR, Human healthcare, General science

Factors affecting fluorophore performance in qPCR

Posted on Wed, Jan 11, 2012 @ 11:00 AM

Fluorophores are molecules that can absorb light energy in a process called excitation and re-emit light energy at a ...
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Topics: qPCR, Human healthcare, General science

Fluorescence Quenching Mechanisms for Dual-Labeled Probes

Posted on Wed, Jun 29, 2011 @ 09:44 AM

A "Referral from the Doctor" Blog Article- Fluorescent probes are used in biochemical assays to monitor specific events ...
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Topics: qPCR, Human healthcare, General science

Multiplexing with Xanthenes

Posted on Wed, Jun 08, 2011 @ 11:13 AM

A "Referral from the Doctor" Blog Article- Quantitative real-time PCR (qPCR) was developed as a means to detect the ...
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Topics: qPCR, Human healthcare, General science

qPCR troubleshooting: interpreting amplification curves and diagnosing problems

Posted on Thu, May 05, 2011 @ 12:15 PM

If you run quantitative real-time PCR (qPCR) assays, the chances are that you’ve seen some strange looking ...
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Topics: qPCR, Human healthcare, General science

Between ROX and a Hard Place

Posted on Wed, Nov 10, 2010 @ 11:12 AM

A "Referral from the Doctor" Blog Article- ROX in qPCR Quantitative real-time RT-PCR was developed as a means of ...
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Topics: qPCR, Human healthcare, General science

Pipetting for qPCR

Posted on Wed, Jun 09, 2010 @ 10:41 AM

A "Referral from the Doctor" Blog Article- Research trends in laboratories today increasingly steer towards gene ...
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Topics: qPCR, Human healthcare, General science

Killer probes for qPCR: what is a Scorpions Primer?

Posted on Wed, Mar 24, 2010 @ 10:57 AM

Scorpions™ Primers are dual-labeled FRET probes that combine a Molecular Beacon-like probe structure and a PCR primer ...
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Topics: qPCR, Human healthcare, General science

A light in the storm: what is a Molecular Beacon?

Posted on Wed, Mar 10, 2010 @ 01:05 PM

Molecular Beacons are a special type of dual-labeled oligonucleotide probe. Beacons are hairpin loop structures with a ...
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Topics: qPCR, Human healthcare, General science

Dual-labeled BHQ probes - Performance to “dye” for

Posted on Wed, Feb 24, 2010 @ 12:20 PM

A "Referral from the Doctor" Blog Article A BHQ® probe is a dual-labeled oligonucleotide covalently labeled with a ...
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Topics: qPCR, Human healthcare, General science

This Year's MVP (Most Valuable Practice): Touchdown PCR

Posted on Thu, Feb 04, 2010 @ 12:39 PM

A "Referral from the Doctor" Blog Article-   The aim of the Polymerase Chain Reaction (PCR) method is to amplify or ...
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Topics: qPCR, Human healthcare, General science

The Grand Ballet: Wine and Biotechnology

Posted on Wed, Jan 27, 2010 @ 01:15 PM

At the third qPCR Symposium, held November 9th -12th in Millbrae, CA, ETS laboratories (ETS) scientist Rich DeScenzo ...
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Topics: qPCR, Human healthcare, General science

Alternative to DNAse I heat inactivation

Posted on Wed, Jan 13, 2010 @ 12:04 PM

A "Referral from the Doctor" Blog Article- Genomic DNA contamination has been a hot topic for qPCR researchers since ...
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Topics: qPCR, Human healthcare, General science

A Quick Jump Start to Multiplexing qPCR

Posted on Wed, Jan 06, 2010 @ 12:01 PM

By now, you've no doubt read about the reductions in error, cost and time that can be achieved by multiplexing ...
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Topics: qPCR, Human healthcare, General science

Slope Efficiency Equals Manageable and Corrected PCR

Posted on Wed, Dec 09, 2009 @ 02:01 PM

A "Referral from the Doctor" Blog Article-   ε = m.c.2 (shall I explain? slope efficiency equals manageable and ...
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Topics: qPCR, Human healthcare, General science

Bringing Back a Champion: Competitive Quantitative Real-time RT-PCR

Posted on Wed, Nov 18, 2009 @ 10:08 AM

A "Referral from the Doctor" Blog Article- Competitive qRT-PCR has become a lost art. Early titans of probe-based PCR ...
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Topics: qPCR, Human healthcare, General science

The New Favorite Reference Gene: 36B4, a.k.a. RPLP0

Posted on Wed, Oct 14, 2009 @ 10:06 AM

A "Referral from the Doctor" Blog Article- Ribosomes are comprised of two subunits, one small (40S) and one large ...
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Topics: Oligo synthesis and modifications, qPCR, Human healthcare, General science

What Genes are Hot, What Genes are Not

Posted on Wed, Oct 07, 2009 @ 04:41 PM

 A "Referral from the Doctor" Blog Article-  Top 10 Reference Genes for probe-based Real-time PCR in 2009: • Acidic ...
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Topics: qPCR, Human healthcare, General science

Labeling oligonucleotides with internal BHQ dyes

Posted on Tue, Sep 29, 2009 @ 10:16 AM

  When to use an internal Black Hole Quencher (BHQ) label in a fluorescence-quenched probe for real-time qPCR? When the ...
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Topics: Oligo synthesis and modifications, qPCR, Human healthcare, General science

Salt-Free Primer Pair Option Now Available

Posted on Tue, Sep 22, 2009 @ 10:08 AM

Biosearch Technologies will now be offering the option of salt-free primer pairs for purchase with any probe order. A ...
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Topics: Oligo synthesis and modifications, qPCR, Human healthcare, General science

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About LGC, Biosearch Technologies

LGC, Biosearch Technologies is the complete Genomics portfolio from LGC. Providing genomic analysis tools, instrumentation and services to the genomic scientific discovery sector worldwide, with focus on across ag bio, pharma and molecular diagnostics. Visit our home page to view our products and services.

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