Updated : Thu, November 16, 2023 @ 3:36 PM
Originally published : Tue, October 3, 2023 @ 10:50 AM
- Bringing oligo synthesis in-house
- Selecting the right instrument
- Selecting the right reagents
- Post-synthesis considerations
Listen to more oligo synthesis advice from nucleic acid synthesis expert Lina Borozdina in this webinar.
Q: How can we start oligo synthesis in a basic lab?
- Select the appropriate instrument to meet your projected needs.
- Ensure you have enough space for the instrument and the required ventilation system.
- Ensure you have proper organic solvent storage and disposal systems.
- Verify that your proposed setup will comply with local fire codes.
- Obtain permits for controlled substances, if required.
- Ensure you have the required accessories.
- Verify that you have other necessary equipment.
- List the types of oligos you will be synthesising and identify supplier partners that can reliably deliver the raw materials at the scales you need.
Q: What are the benefits of synthesising your own oligos?
Q: Can LGC Biosearch Technologies help scale up manufacturing if we develop the initial oligos ourselves?
Q: How much training is required to operate your MerMade oligo instrument?
Q: What kind of support is available to customers who have purchased a MerMade oligo synthesizer from your company?
Q: Is there a need to clean the oligo synthesizer between two synthesis rounds, and if so, how time-consuming is the process?
Q: Are there limitations on the length of oligos that your machines can synthesise?
Q: How can a 6-oligo machine produce 6 oligos and a 96-oligo machine produce 96 oligos in the same amount of time?
Q: I need to synthesise oligos ranging from 20 to 60 nucleotides. Which oligo synthesizer would you recommend?
|Oligo synthesizer||Throughput||Columns format||Max amidite positions (standard)||Columns format||Scale||Benefit|
|MerMade 4||low||4||10||20mer primers in 3.5 hours on each column||50 nmol to 5 μmol||Each of the four columns can be assigned a different protocol to synthesise oligos of different quality and yield within the same run.|
|MerMade 6||medium||6||10, 16, 20 and 24||20mer primers in 3.5 hours on each column||50 nmol to 200 μmol||Capable of making a combination of standard, degenerate and modified oligos in the same run. Unmatched flexibility, upgrade ability, dependability, efficiency and ease of use.|
|MerMade 12||medium||12||10, 16, 20 and 24||20mer primers in 3.5 hours on each column||50 nmol to 200 μmol||Ideal for labs requiring medium throughput with maximum flexibility. Capable of making a combination of standard, degenerate and modified oligos in the same run.|
|MerMade 48X||high||4 plates of 12 columns||20 or 24||20mer primers in 3.5 hours||50 nmol to 5 μmol||A fast oligo synthesizer that is capable of producing high quality oligos – even those with extensive modifications. Allows for hot swapping plates as the synthesis is completed to facilitate a continuous, efficient operation.|
|MerMade 96E||high||96 well format of columns||12||20mer primers in 3.5 hours||50 nmol to 1 μmol||Ideally suited for labs that need a fast synthesizer that can be configured for all types of chemistry and is capable of producing high-quality oligos.|
|MerMade 192E||high||2x 96 well format of columns||8||20mer primers in 3.5 hours||50 nmol to 5 μmol||Produces a very high-quality product with very minimal reagent usage. Ideal for those who need a fast synthesizer that is capable of producing high-quality oligos that do not require extensive modifications.|
|MerMade 192X||high||2x 96 well format of columns||12-64||20mer primers in 3.5 hours||50 nmol to 5 μmol||This oligo synthesizer adds even more flexibility, configurability and speed compared to models listed above. It can be configured to synthesise all types of chemistries and produces high-quality oligos.|
Explore the full range of oligo synthesizers from Biosearch Technologies.