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Updated : Thu, June 1, 2023 @ 11:18 AM
Originally published : Thu, Feb 26, 2015 @ 04:00 PM
Updated : Thu, June 1, 2023 @ 11:18 AM
Originally published : Thu, Feb 26, 2015 @ 04:00 PM
Buffers are often overlooked and taken for granted until the day comes when a peculiar result is observed and its origin is traced to a bad buffer. Although in very rare cases mistakes in the composition of buffers have led to discoveries such as the correct number of human chromosomes1, using properly prepared buffers can be the key to success with technologies such as Stellaris RNA FISH.
Buffers are employed for any experiment that requires the structure and/or activity of biological material to be maintained. They function to resist changes in hydrogen ion concentrations and to provide critical salt concentrations, nutrients (such as for cell culture), and co-factors required for enzymatic reactions to take place. The new Stellaris RNA FISH Buffers come with 4 major advantages:
*View a hi-res version of the Stellaris FISH Buffers comparison
Fig - A direct comparison showing background reduction by utilizing Stellaris RNA FISH Buffers (Cat#: SMF-HB1-10, SMF-WA1-60, SMF-WB1-20). Following the adherent cell protocol, formalin fixed human cells were probed with Human KRAS, Human POLR2A (Cat# VSMF-2293-5) or Human TOP1 (Cat# VSMF-2419-5). Left Panel (A,B,C): RNA FISH performed with Stellaris RNA FISH Buffers. Right Panel (D,E,F): RNA FISH performed with Conventional RNA FISH buffer recipes. Note: Results may vary according to original probe set performance and sample type.
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