Multiplex your qPCR and Walk-away

Updated : Mon, September 19, 2022 @ 2:15 PM

It seems everyone wants to do more with less, and automate the process as well.  But this can cause a number of challenges for even a traditional molecular diagnostics tool such as PCR.The IntelliQube by LGC Douglas Scientific

The versatility of PCR has led to a myriad of spin-off techniques, including probe-based quantitative PCR (qPCR). To increase the utility of the method, multiplexed qPCR uses several pairs of gene-specific primers and probes to amplify and measure multiple target sequences simultaneously. This helps reduce material costs and increase efficiencies, although upstream optimisation is required.

While there are a number of PCR instruments on the market that can perform one-step qPCR , the IntelliQube® is unique in providing a fully-automated walk-away system that delivers the flexibility and scalability necessary to economically expand qPCR workflows while minimising reaction volumes of just 1.6 uL using Array Tape®.

The IntelliQube by LGC Douglas ScientificWe recently worked with experts from TATAA Biocenter in Sweden to evaluate the performance of the IntelliQube using BHQ-probe based assays for a gene expression model study of the Mucin 1 (Muc1) gene across a number of tissues types.

Without wanting to provide too many spoilers, the study demonstrates the ability of the instrument to successfully multiplex three BHQ qPCR assays and a passive reference dye for gene expression studies without compromising data quality.


To learn more, just click here to download the study.

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LGC, Biosearch Technologies is the complete Genomics portfolio from LGC. Providing genomic analysis tools, instrumentation and services to the genomic scientific discovery sector worldwide, with focus on across ag bio, pharma and molecular diagnostics. Visit our home page to view our products and services.

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