The BiosearchTech Blog

Best practices for implementing targeted genotyping by sequencing

Posted on Wed, Dec 02, 2020 @ 08:53 AM

In breeding programmes, selections based on a single or small number of markers to target traits in marker assisted selection (MAS) often miss the contributions of a large proportion of the genetic variants or small effects from small loci, which require additional breeding cycles and selections to identify the best lines to take forward. Traditional genotyping data from PCR and microsatellites or single sequence repeats (SSRs) doesn’t provide the details of variation at the sequence level while the fixed nature of arrays prevents them from being adapted to varying accessions, populations and breeding strategies.

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Topics: Genomics, AgBio, Genomic Selection, Marker Assisted Selection, Targeted Genotyping, Targeted Genotyping by Sequencing

Building resilience into SARS-CoV-2 testing workflows: considerations for choosing an RNA isolation method

Posted on Wed, Oct 21, 2020 @ 09:00 AM

As we start to close in on a full year of the COVID-19 pandemic, molecular diagnostics labs are still facing supply chain challenges with their SARS-CoV-2 testing workflows, and these shortages are only expected to get worse as the Northern Hemisphere flu season gets underway. These challenges limit testing volume and delay the delivery of results that help individuals make decisions on quarantining, working, and traveling, and are essential for keeping communities safe as they navigate a return to normalcy. 

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Topics: Molecular Diagnostics, Genomics, Coronavirus

How high-throughput genotyping automation and KASP marker libraries connect research labs with commercial breeders to accelerate crop improvement

Posted on Wed, Oct 07, 2020 @ 09:31 AM

“In July of 2015, I was a member of the radish genome project team. My task was to examine genetic diversity of radish cultivars. Although we did find tons of SNP information from genome sequence data, we didn’t have a cost-effective method for custom SNP genotyping. So I had to design Indel markers and applied them to radish accessions. While I was preparing PCR reactions of thirty 96-well plates, I felt that it’s too ironic to do this sluggish work in the era of NGS and genome. It compelled me to take this picture of the experiment. The PCR reactions and gel electrophoresis took more than two weeks. It was quite laborious work. Even after the job was done, the stark reality of using labor-intensive methods never left my mind, which is what probably led me to my current job.”

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Topics: Genomics, Genotyping, KASP

Employee spotlight: Meet Darshna "Dusty" Vyas

Posted on Mon, Sep 14, 2020 @ 08:15 AM

Darshna "Dusty" Vyas has been a Senior Scientist at LGC specialising in plant genetics for the past 8 years Her contributions on the team have enabled the translation of LGC, Biosearch Technologies to the global plant breeding community in all species ranging across diverse sample types including wheat, cocoa, rubber, rice and aquaculture. Dusty's desire to understand our customers’ requirements and develop innovative products and services at Biosearch Technologies to improve global crop development isn't just a professional objective, but a personal one. 

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Topics: Agrigenomics, Genomics, KASP

Pressing COVID-19 testing issues of today and the near future

Posted on Wed, Aug 26, 2020 @ 07:57 AM

Following up with the panelists from our recent COVID-19 webinar, Part 3 of 3

In the previous two blog posts in this series, we shared insights from two of the panelists from our webinar, “How to establish COVID-19 diagnostics and keep up with demand,” covering testing scale up and issues related to sensitivity and accuracy. In this final post of the series, we ask our panelists to address questions about some of the most pressing issues of today and the near future.

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Topics: Molecular Diagnostics, Genomics, Coronavirus

Sensitivity and accuracy in COVID-19 testing

Posted on Wed, Aug 19, 2020 @ 07:56 AM

Following up with the panelists from our recent COVID-19 webinar, Part 2 of 3

In the face of a rapidly evolving pandemic, testing labs are constantly having to adjust and adapt their existing workflows. In our recent webinar, “How to establish COVID-19 diagnostics and keep up with demand,” we assembled a panel of experts to share insights that can help testing labs find a path forward. In this continuation of our webinar follow-up conversation with panelists Eric Chow, PhD, and Kathleen Campau, PhD, we discuss questions from the webinar audience related to SARS-CoV-2 testing sensitivity and accuracy.

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Topics: Molecular Diagnostics, Genomics, Coronavirus

Scaling up COVID-19 viral testing

Posted on Wed, Aug 12, 2020 @ 07:47 AM

Following up with the panelists from our recent COVID-19 webinar, Part 1 of 3

The COVID-19 pandemic has presented testing labs around the world with a number of new challenges, and the community is still working out the best ways to meet the incredible testing need. To help labs continue to refine and improve their SARS-CoV-2 testing processes and workflows, LGC, Biosearch Technologies recently held an information-rich webinar, 'How to establish COVID-19 diagnostics and keep up with demand', with a panel of experts from both industry and academia. Here we follow-up with two of the panelists, Eric Chow, PhD, and Kathleen Campau, PhD, with additional questions from the audience on scaling up. 

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Topics: Molecular Diagnostics, Genomics, Coronavirus

Enabling a rapid response to COVID-19 by Honeywell and LGC, Biosearch Technologies

Posted on Wed, Jul 29, 2020 @ 12:40 PM

As countries worldwide begin to ease lockdown restrictions during the pandemic and prepare to return to “normal” without a vaccine for COVID-19 or effective treatments in place, prudent testing measures need to be taken to suppress new infections. It is critical to test more people rapidly to identify who is infected, track them to avoid the disease from spreading and tracing with whom they have been in contact. Reverse transcription PCR (RT-PCR) testing method currently represents the majority of COVID-19 molecular diagnostics that helps identify individuals who are infected at the time of the test.

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Topics: Molecular Diagnostics, Genomics, qPCR, Coronavirus

Establishment of KASP marker library for genetic diversity analysis in Brassica rapa  crops

Posted on Tue, Jul 14, 2020 @ 08:17 AM

Brassica  rapa crops are important sources of food, feed, and fuel in many parts of the world and provide a unique opportunity for investigating plant genome evolution. In 2018, the Food and Agricultural Organization reported a global production of cabbages and other brassicas to be more than 103 million tonnes.1 Disease and environmental stress can reduce crop yield and quality, so plant breeders are using genome-wide molecular markers like SNPs to make a background selection and identify target genes involved in high nutritional quality, disease resistance and yield in order to speed up the breeding process in Brassica rapa crops. 

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Topics: Agrigenomics, Genomics, KASP

Robust supply chains and open testing systems: How our AgBio roots enable solutions to the COVID-19 pandemic

Posted on Tue, May 19, 2020 @ 08:18 AM

It may seem strange that a company known for transforming the global seed breeding industry is uniquely positioned to help COVID-19 testing labs overcome bottlenecks and rapidly scale-up testing. However, when you look at the problems testing labs are facing, it becomes clear that LGC, Biosearch Technologies has all the right tools for a rapid and robust pandemic response, even with the need to validate our Research Use Only (RUO) products for diagnostic testing. Today’s significantly altered conditions require new approaches and new solutions.

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Topics: Molecular Diagnostics, Genomics, qPCR, Coronavirus

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About LGC, Biosearch Technologies

LGC, Biosearch Technologies is the complete Genomics portfolio from LGC. Providing genomic analysis tools, instrumentation and services to the genomic scientific discovery sector worldwide, with focus on across ag bio, pharma and molecular diagnostics. Visit our home page to view our products and services.

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