What Genes are Hot, What Genes are Not

Updated : Mon, September 19, 2022 @ 2:10 PM 

A "Referral from the Doctor" Blog Article-

 Top 10 Reference Genes for probe-based Real-time PCR in 2009:

• Acidic Ribosomal Phosphoprotein P0 (RPLP0 or 36B4)
• ATP-synthase subunit 5B (A5B)
• Tumor Protein Translationally controlled 1 (TPT1)
• Signal Recognition Particle 14kDa (SRP14)
• TATA-Binding Protein (TBP)
• Eukaryotic Elongation Factor 1A1 (EEF1A1)
• Hypoxanthine Phosphoribosyl-Transferase 1 (HPRT1)
• poly-Ubiquitin (Ubi)
• Glyceraldehyde-3-phosphate dehydrogenase (G3PD)
• Beta-actin (ACTB)

See next week's blog for follow up information!

Written by: Christina Ferrell, Ph.D., Technical Applications Specialist

A. Pilbrow, L. Ellmers, M. Black, C. Moravec, W. Sweet, R. Troughton, A.M. Richards, C. Frampton, V. Cameron. Genomic selection of reference genes for real-time PCR in human myocardium. BMC Medical Genomics (2008) 1(1):64

Li-Yun Fu, Hu-Liang Jia, Qiong-Zhu Dong, Jin-Cai Wu, Yue Zhao, Hai-Jun Zhou, Ning Ren, Qin-Hai Ye, Lu-Xiu Qin. Suitable reference genes for real-time PCR in human HBV-related hepatocellular carcinoma with different clinical prognoses. BMC Cancer (2009) 9:49

E.D. Silveira, M. Alves-Ferreira, L.A. Guimaraes, F. R. da Silva, V. T. de Campos Carneiro. Selection of reference genes for quantitative real-time PCR expression studies in apomictic and sexual grass Brachiaria brizantha. BMC Plant Biology (2009) 9:84

G. Silberberg, K. Baruch, R. Navon. Detection of stable reference genes for real-time PCR analysis in schizophrenia and bipolar disorder. Analytical Biochemistry (2009) 391(2):91-97

B. Etschmann, B. Wilcken, K. Stoevesand, A. von der Schulenburg, A. Sterner-Kock. Selection of reference genes for qualitative real-time PCR analysis in canine mammary tumors using geNorm algorithm. Vet Pathol. (2006) 43:934-942

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