Stellaris® RNA FISH (fluorescence in situ hybridization) is an RNA visualization method that allows simultaneous detection, localization, and quantification at the sub-cellular level in fixed samples using widefield or confocal fluorescence microscopy.
Recently, LGC's R&D Scientist Dr. Hans Johansson presented at the Helen Diller Cancer Institute at UCSF highlighting the basic chemistries and various downstream applications of Stellaris RNA FISH and how this technology can be used to support the oncology research being conducted at UCSF.
During the presentation Dr. Johansson also discusses:
specifics of designing Stellaris probe sets and how to validate for target specificity
compatibility with immunofluorescence (IF) and multiplexing multiple RNA targets
characterizing cell lines, disease phenotypes, allelic specific expression, transcription bursts, variant abundance, knock down, induced expression, nuclear RNAs and localization
If you weren't able to attend, or if you'd just like to catch it again, watch the presentation in its entirety below:
Interested in learning more about how RNA FISH is being used for cancer targets? Head on over to this page.