What Genes are Hot, What Genes are Not

Updated : Mon, September 19, 2022 @ 2:10 PM 

A "Referral from the Doctor" Blog Article-

 Top 10 Reference Genes for probe-based Real-time PCR in 2009:

• Acidic Ribosomal Phosphoprotein P0 (RPLP0 or 36B4)
• ATP-synthase subunit 5B (A5B)
• Tumor Protein Translationally controlled 1 (TPT1)
• Signal Recognition Particle 14kDa (SRP14)
• TATA-Binding Protein (TBP)
• Eukaryotic Elongation Factor 1A1 (EEF1A1)
• Hypoxanthine Phosphoribosyl-Transferase 1 (HPRT1)
• poly-Ubiquitin (Ubi)
• Glyceraldehyde-3-phosphate dehydrogenase (G3PD)
• Beta-actin (ACTB)

See next week's blog for follow up information!

Written by: Christina Ferrell, Ph.D., Technical Applications Specialist

References:
A. Pilbrow, L. Ellmers, M. Black, C. Moravec, W. Sweet, R. Troughton, A.M. Richards, C. Frampton, V. Cameron. Genomic selection of reference genes for real-time PCR in human myocardium. BMC Medical Genomics (2008) 1(1):64

Li-Yun Fu, Hu-Liang Jia, Qiong-Zhu Dong, Jin-Cai Wu, Yue Zhao, Hai-Jun Zhou, Ning Ren, Qin-Hai Ye, Lu-Xiu Qin. Suitable reference genes for real-time PCR in human HBV-related hepatocellular carcinoma with different clinical prognoses. BMC Cancer (2009) 9:49

E.D. Silveira, M. Alves-Ferreira, L.A. Guimaraes, F. R. da Silva, V. T. de Campos Carneiro. Selection of reference genes for quantitative real-time PCR expression studies in apomictic and sexual grass Brachiaria brizantha. BMC Plant Biology (2009) 9:84

G. Silberberg, K. Baruch, R. Navon. Detection of stable reference genes for real-time PCR analysis in schizophrenia and bipolar disorder. Analytical Biochemistry (2009) 391(2):91-97

B. Etschmann, B. Wilcken, K. Stoevesand, A. von der Schulenburg, A. Sterner-Kock. Selection of reference genes for qualitative real-time PCR analysis in canine mammary tumors using geNorm algorithm. Vet Pathol. (2006) 43:934-942