How do SARS-CoV-2 variants affect PCR assays for COVID-19 testing?

How do SARS-CoV-2 variants affect PCR assays for COVID-19 testing?

Researchers have identified several variants of SARS-CoV-2 since they sequenced the original viral strain responsible for the COVID-19 pandemic from clinical samples in Wuhan, China. Four variants, UK (B1.1.7), B1.1.7 +E484K, South African (B1.351, 501v2) and Brazilian (B1.1.28, P1), are of particular concern. The United States Center for Disease Control and Prevention (CDC) warns that these variants have the potential to...

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RT-qPCR and the Stellaris FISH Method

Posted on Fri, Apr 26, 2013 @ 10:32 AM

A "Referral from the Doctor" Blog Article- Quantitative reverse transcription polymerase chain reaction (RT-qPCR) is a ...
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Topics: Real-time PCR, RNA FISH, Referral from the Doctor

Factors affecting fluorophore performance in qPCR

Posted on Wed, Jan 11, 2012 @ 09:00 AM

A "Referral from the Doctor" Blog Article- In qPCR, fluorescent modified oligos may be either linear or non-linear in ...
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The 'Monte Carlo' Effect in qPCR

Posted on Wed, Nov 09, 2011 @ 11:00 AM

A "Referral from the Doctor" Blog Article- The mathematical model of PCR is based on the assumption that each DNA ...
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Fluorescence Quenching Mechanisms for Dual-Labeled Probes

Posted on Wed, Jun 29, 2011 @ 07:44 AM

A "Referral from the Doctor" Blog Article- Fluorescent probes are used in biochemical assays to monitor specific events ...
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Multiplexing with Xanthenes

Posted on Wed, Jun 08, 2011 @ 09:13 AM

A "Referral from the Doctor" Blog Article- Quantitative real-time PCR (qPCR) was developed as a means to detect the ...
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Between ROX and a Hard Place

Posted on Wed, Nov 10, 2010 @ 09:12 AM

A "Referral from the Doctor" Blog Article- ROX in qPCR Quantitative real-time RT-PCR was developed as a means of ...
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Taq Facts

Posted on Tue, Aug 03, 2010 @ 08:14 AM

A "Referral from the Doctor" Blog Article- When the polymerase chain reaction (PCR) was first described, the Klenow ...
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Pipetting for qPCR

Posted on Wed, Jun 09, 2010 @ 08:41 AM

A "Referral from the Doctor" Blog Article- Research trends in laboratories today increasingly steer towards gene ...
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Black Hole Scorpion® Primers - Killer probes for qPCR

Posted on Wed, Mar 24, 2010 @ 08:57 AM

A "Referral from the Doctor" Blog Article-   Scorpions®Primers are dual-labeled FRET probes that combine a Molecular ...
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Molecular Beacons - Lights in the storm

Posted on Wed, Mar 10, 2010 @ 11:05 AM

A "Referral from the Doctor" Blog Article- Molecular Beacons are a special type of dual-labeled oligonucleotide probe. ...
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Dual-labeled BHQ probes - Performance to “dye” for

Posted on Wed, Feb 24, 2010 @ 10:20 AM

A "Referral from the Doctor" Blog Article-   A BHQ® probe is a dual-labeled oligonucleotide covalently labeled with a ...
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This Year's MVP (Most Valuable Practice): Touchdown PCR

Posted on Thu, Feb 04, 2010 @ 10:39 AM

A "Referral from the Doctor" Blog Article-   The aim of the Polymerase Chain Reaction (PCR) method is to amplify or ...
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An Alternative to DNAse I heat inactivation: LiCl precipitation

Posted on Wed, Jan 13, 2010 @ 10:04 AM

A "Referral from the Doctor" Blog Article- Genomic DNA contamination has been a hot topic for qPCR researchers since ...
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Slope Efficiency Equals Manageable and Corrected PCR

Posted on Wed, Dec 09, 2009 @ 12:01 PM

A "Referral from the Doctor" Blog Article-   ε = m.c.2 (shall I explain? slope efficiency equals manageable and ...
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Bringing Back a Champion: Competitive Quantitative Real-time RT-PCR

Posted on Wed, Nov 18, 2009 @ 08:08 AM

A "Referral from the Doctor" Blog Article- Competitive qRT-PCR has become a lost art. Early titans of probe-based PCR ...
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The New Favorite Reference Gene: 36B4, a.k.a. RPLP0

Posted on Wed, Oct 14, 2009 @ 08:06 AM

A "Referral from the Doctor" Blog Article- Ribosomes are comprised of two subunits, one small (40S) and one large ...
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What Genes are Hot, What Genes are Not

Posted on Wed, Oct 07, 2009 @ 02:41 PM

 A "Referral from the Doctor" Blog Article-  Top 10 Reference Genes for probe-based Real-time PCR in 2009: • Acidic ...
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