When the polymerase chain reaction (PCR) was first described, the Klenow fragment of Escherichia coli DNA Polymerase I ...
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Choosing the best qPCR probe chemistry for your assay
There are many different chemistries available for qPCR probes offering scientists a wealth of options during assay development. However, the unique chemistry of each probe type means that you’ll get the best assay performance when you match the probe type to the application.
Read on to find out more about the chemistries in our industry-leading probe portfolio and what makes each of them shine.
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A recent study coordinated by LGC and published in the journal Analytical Chemistry, has demonstrated the potential of ...
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Topics: Real-time PCR
As the Zika virus outbreak spreads, a wave of international concern has grown, and scientists have scrambled to ...
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Topics: Real-time PCR
OLIGO SYNTHESIS PHOTO GLIMPSE We’ve recently captured our talented manufacturing teams hard at work building oligos. ...
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Topics: Molecular Diagnostics, Real-time PCR, Oligo synthesis and modifications
LGC Biosearch Technologies has developed a new qPCR probe format powered by Black Hole Quencher® technology known as ...
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Topics: Real-time PCR
This week, we continue our Know your oligo mod blog series and we will focus on a very basic, yet extremely versatile ...
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Studying the expression level of RNA transcripts is often complicated by the fact that many DNA sequences are ...
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Topics: Real-time PCR, RNA FISH
Since its inception, qPCR has been recognized as a powerful molecular method. However, the application of this ...
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Topics: Real-time PCR
In the setup of a qPCR experiment, one of the first decisions to make is which detection method to use. This generally ...
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Topics: Real-time PCR
In a recent webinar, Dr. James Willey describes a two color fluorometric assay for more robust quantitation of nucleic ...
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Topics: Real-time PCR
Single Nucleotide Polymorphisms (SNPs) have been widely studied for over 15 years, and have applications in forensics, ...
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Topics: Real-time PCR, PCR-based genotyping
Biosearch Technologies has launched qPCRdesign.com, a new microsite targeted specifically for your quantitative PCR ...
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Topics: Real-time PCR
Different oligonucleotide manufacturers deliver probes and primers in a variety of different measured units including ...
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The largest recorded Ebola Virus Disease outbreak in history continues to devastate the West African region. This ...
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Topics: Molecular Diagnostics, Real-time PCR
Don’t waste time performing an extra step. Cut back on pipetting and order a ValuMix™ Assay to have your probes and ...
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Topics: Real-time PCR
Do you remember when the most popular method of DNA amplification involved multiple hybridizations, followed by single ...
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Topics: Real-time PCR
A "Referral from the Doctor" Blog Article- Quantitative reverse transcription polymerase chain reaction (RT-qPCR) is a ...
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Topics: Real-time PCR, RNA FISH
Non-squamous, Non-small cell lung cancer (NSCLC), the most common cause of death in the US and globally, has recently ...
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Topics: Molecular Diagnostics, Real-time PCR
A "Referral from the Doctor" Blog Article- Fluorescent probes are used in biochemical assays to monitor specific events ...
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Topics: Real-time PCR
A "Referral from the Doctor" Blog Article- Quantitative real-time PCR (qPCR) was developed as a means to detect the ...
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Topics: Real-time PCR
If you run quantitative real-time PCR (qPCR) assays, the chances are that you’ve seen some strange looking ...
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Topics: Real-time PCR
A "Referral from the Doctor" Blog Article- ROX in qPCR Quantitative real-time RT-PCR was developed as a means of ...
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Topics: Real-time PCR
Biosearch Technologies today announces that it has entered into a new license relationship with QIAGEN (NASDAQ: QIA, ...
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Topics: Real-time PCR
A "Referral from the Doctor" Blog Article- Research trends in laboratories today increasingly steer towards gene ...
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Topics: Real-time PCR
A "Referral from the Doctor" Blog Article- Scorpions®Primers are dual-labeled FRET probes that combine a Molecular ...
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Topics: Real-time PCR
A "Referral from the Doctor" Blog Article- Molecular Beacons are a special type of dual-labeled oligonucleotide probe. ...
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Topics: Real-time PCR
A "Referral from the Doctor" Blog Article- A BHQ® probe is a dual-labeled oligonucleotide covalently labeled with a ...
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Topics: Real-time PCR
A "Referral from the Doctor" Blog Article- The aim of the Polymerase Chain Reaction (PCR) method is to amplify or ...
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Topics: Real-time PCR
At the third qPCR Symposium, held November 9th -12th in Millbrae, CA, ETS laboratories (ETS) scientist Rich DeScenzo ...
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Topics: Real-time PCR
A "Referral from the Doctor" Blog Article- Genomic DNA contamination has been a hot topic for qPCR researchers since ...
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Topics: Real-time PCR
By now, you've no doubt read about the reductions in error, cost and time that can be achieved by multiplexing ...
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Topics: Real-time PCR
A "Referral from the Doctor" Blog Article- ε = m.c.2 (shall I explain? slope efficiency equals manageable and ...
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Topics: Real-time PCR
A "Referral from the Doctor" Blog Article- Competitive qRT-PCR has become a lost art. Early titans of probe-based PCR ...
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Topics: Real-time PCR
A "Referral from the Doctor" Blog Article- Ribosomes are comprised of two subunits, one small (40S) and one large ...
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A "Referral from the Doctor" Blog Article- Top 10 Reference Genes for probe-based Real-time PCR in 2009: • Acidic ...
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Topics: Real-time PCR
When to use an internal Black Hole Quencher (BHQ) label in a fluorescence-quenched probe for real-time qPCR? When the ...
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Biosearch Technologies will now be offering the option of salt-free primer pairs for purchase with any probe order. A ...
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