Considerations for RT-qPCR in molecular point of care testing devices

Considerations for RT-qPCR in molecular point of care testing devices

The COVID-19 pandemic has accelerated the uptake of point of care testing (POCT), with simple, user-administered tests becoming commonplace in schools, places of work and people’s homes. Antigen POCT has been pivotal in SARS-CoV-2 detection, particularly where reverse transcription PCR (RT-PCR) cannot be delivered in a timely manner. However, the use of antigen tests isn’t always appropriate due to variable sensitivity. RT-PCR – or RT-qPCR if using real-time detection – offers greater sensitivity and specificity. Leveraging this technology in POCT brings lab quality results to the point of need, providing rapid, accurate and actionable results to clinicians.

Read more

The BiosearchTech Blog

Most popular

Digital PCR – improving reproducible molecular methods in the clinic

Posted on Thu, Apr 06, 2017 @ 08:40 AM

A recent study coordinated by LGC and published in the journal Analytical Chemistry, has demonstrated the potential of ...
Read more

Topics: Real-time PCR

qPCR-based Molecular Diagnostics at the Forefront of Zika Surveillance

Posted on Tue, Aug 23, 2016 @ 02:00 PM

As the Zika virus outbreak spreads, a wave of international concern has grown, and scientists have scrambled to ...
Read more

Topics: Real-time PCR

Photoblog: Behind the scenes of oligo synthesis

Posted on Wed, May 18, 2016 @ 03:00 PM

OLIGO SYNTHESIS PHOTO GLIMPSE We’ve recently captured our talented manufacturing teams hard at work building oligos. ...
Read more

Topics: Molecular Diagnostics, Real-time PCR, Oligo synthesis and modifications

Nova is the new black

Posted on Thu, Apr 14, 2016 @ 02:00 PM

LGC Biosearch Technologies has developed a new qPCR probe format powered by Black Hole Quencher® technology known as ...
Read more

Topics: Real-time PCR

Introducing BHQnova Probes for qPCR - Join the Early Access Program

Posted on Mon, Feb 29, 2016 @ 02:00 PM

LGC Biosearch Technologies is announcing the upcoming release of a new qPCR probe format known as BHQnova™ probes. This ...
Read more

Topics: Real-time PCR

Know Your Oligo Mod: 3' Spacer C3

Posted on Thu, Oct 15, 2015 @ 02:15 PM

This week, we continue our Know Your Oligo Mod series and we will focus on a very basic, yet extremely versatile ...
Read more

Topics: Real-time PCR, Oligo synthesis and modifications

And the Consensus (Sequence) is...

Posted on Thu, Jun 11, 2015 @ 02:31 PM

Studying the expression level of RNA transcripts is often complicated by the fact that many DNA sequences are ...
Read more

Topics: Real-time PCR, RNA FISH

MIQE Goes Digital with Open Access qPCR Guide

Posted on Thu, May 21, 2015 @ 02:00 PM

Since its inception, qPCR has been recognized as a powerful molecular method. However, the application of this ...
Read more

Topics: Real-time PCR

How Much is Your qPCR Assay Really Costing You?

Posted on Thu, Apr 02, 2015 @ 02:00 PM

In the setup of a qPCR experiment, one of the first decisions to make is which detection method to use. This generally ...
Read more

Topics: Real-time PCR

BHQplus Probes Optimize Multiplex Competitive qRT-PCR in Diagnostics

Posted on Thu, Jan 29, 2015 @ 02:00 PM

In a recent webinar, Dr. James Willey describes a two color fluorometric assay for more robust quantitation of nucleic ...
Read more

Topics: Real-time PCR

A Successful Duo for Accurate, Speedy, and Economical SNP Genotyping

Posted on Thu, Jan 08, 2015 @ 11:30 AM

Single Nucleotide Polymorphisms (SNPs) have been widely studied for over 15 years, and have applications in forensics, ...
Read more

Topics: Real-time PCR, PCR-based genotyping

Check out our new website qPCR Design Lab @ qPCRdesign.com

Posted on Thu, Oct 23, 2014 @ 02:00 PM

Biosearch Technologies has launched qPCRdesign.com, a new microsite targeted specifically for your quantitative PCR ...
Read more

Topics: Real-time PCR

Delivering Oligos by Nanomole Units

Posted on Wed, Oct 15, 2014 @ 11:00 AM

Different oligonucleotide manufacturers deliver probes and primers in a variety of different measured units including ...
Read more

Topics: Real-time PCR, Oligo synthesis and modifications

RT-qPCR Molecular Diagnostics Assist Workers at the Forefront of Ebola Outbreak.

Posted on Thu, Sep 25, 2014 @ 11:30 AM

The largest recorded Ebola Virus Disease outbreak in history continues to devastate the West African region. This ...
Read more

Topics: Molecular Diagnostics, Real-time PCR

One Step too Many? Simplify your qPCR Protocol.

Posted on Thu, Feb 27, 2014 @ 10:02 AM

Don’t waste time performing an extra step. Cut back on pipetting and order a ValuMix™ Assay to have your probes and ...
Read more

Topics: Real-time PCR

30 Years: The Impact of PCR

Posted on Thu, Sep 05, 2013 @ 09:29 AM

Do you remember when the most popular method of DNA amplification involved multiple hybridizations, followed by single ...
Read more

Topics: Real-time PCR

RT-qPCR and the Stellaris FISH Method

Posted on Fri, Apr 26, 2013 @ 10:32 AM

A "Referral from the Doctor" Blog Article- Quantitative reverse transcription polymerase chain reaction (RT-qPCR) is a ...
Read more

Topics: Real-time PCR, RNA FISH

qPCR Probes at work: Innovative CLIA test for NSCLC staging

Posted on Tue, Mar 06, 2012 @ 11:00 AM

Non-squamous, Non-small cell lung cancer (NSCLC), the most common cause of death in the US and globally, has recently ...
Read more

Topics: Molecular Diagnostics, Real-time PCR

Factors affecting fluorophore performance in qPCR

Posted on Wed, Jan 11, 2012 @ 09:00 AM

A "Referral from the Doctor" Blog Article- In qPCR, fluorescent modified oligos may be either linear or non-linear in ...
Read more

Topics: Real-time PCR

Fluorescence Quenching Mechanisms for Dual-Labeled Probes

Posted on Wed, Jun 29, 2011 @ 07:44 AM

A "Referral from the Doctor" Blog Article- Fluorescent probes are used in biochemical assays to monitor specific events ...
Read more

Topics: Real-time PCR

Multiplexing with Xanthenes

Posted on Wed, Jun 08, 2011 @ 09:13 AM

A "Referral from the Doctor" Blog Article- Quantitative real-time PCR (qPCR) was developed as a means to detect the ...
Read more

Topics: Real-time PCR

qPCR troubleshooting: interpreting amplification curves and diagnosing problems

Posted on Thu, May 05, 2011 @ 10:15 AM

If you run quantitative real-time PCR (qPCR) assays, the chances are that you’ve seen some strange looking ...
Read more

Topics: Real-time PCR

Between ROX and a Hard Place

Posted on Wed, Nov 10, 2010 @ 09:12 AM

A "Referral from the Doctor" Blog Article- ROX in qPCR Quantitative real-time RT-PCR was developed as a means of ...
Read more

Topics: Real-time PCR

Biosearch Technologies and Seegene Finalize Oligonucleotide Supply and BHQ®, CAL Fluor® and Quasar® Licensing Agreements

Posted on Wed, Oct 20, 2010 @ 08:12 AM

Biosearch Technologies, Inc. (Biosearch), a leading supplier of sophisticated oligonucleotide components to the rapidly ...
Read more

Topics: Real-time PCR

Biosearch Technologies Expands Licensing of QIAGEN Scorpions® Patents

Posted on Thu, Aug 12, 2010 @ 08:41 AM

Biosearch Technologies today announces that it has entered into a new license relationship with QIAGEN (NASDAQ: QIA, ...
Read more

Topics: Real-time PCR

Taq facts

Posted on Tue, Aug 03, 2010 @ 08:14 AM

When the polymerase chain reaction (PCR) was first described, the Klenow fragment of Escherichia coli DNA Polymerase I ...
Read more

Topics: Real-time PCR, qPCR

Pipetting for qPCR

Posted on Wed, Jun 09, 2010 @ 08:41 AM

A "Referral from the Doctor" Blog Article- Research trends in laboratories today increasingly steer towards gene ...
Read more

Topics: Real-time PCR

Black Hole Scorpion® Primers - Killer probes for qPCR

Posted on Wed, Mar 24, 2010 @ 08:57 AM

A "Referral from the Doctor" Blog Article-   Scorpions®Primers are dual-labeled FRET probes that combine a Molecular ...
Read more

Topics: Real-time PCR

Molecular Beacons - Lights in the storm

Posted on Wed, Mar 10, 2010 @ 11:05 AM

A "Referral from the Doctor" Blog Article- Molecular Beacons are a special type of dual-labeled oligonucleotide probe. ...
Read more

Topics: Real-time PCR

Dual-labeled BHQ probes - Performance to “dye” for

Posted on Wed, Feb 24, 2010 @ 10:20 AM

A "Referral from the Doctor" Blog Article-   A BHQ® probe is a dual-labeled oligonucleotide covalently labeled with a ...
Read more

Topics: Real-time PCR

This Year's MVP (Most Valuable Practice): Touchdown PCR

Posted on Thu, Feb 04, 2010 @ 10:39 AM

A "Referral from the Doctor" Blog Article-   The aim of the Polymerase Chain Reaction (PCR) method is to amplify or ...
Read more

Topics: Real-time PCR

The Grand Ballet: Wine and Biotechnology

Posted on Wed, Jan 27, 2010 @ 11:15 AM

At the third qPCR Symposium, held November 9th -12th in Millbrae, CA, ETS laboratories (ETS) scientist Rich DeScenzo ...
Read more

Topics: Real-time PCR

An Alternative to DNAse I heat inactivation: LiCl precipitation

Posted on Wed, Jan 13, 2010 @ 10:04 AM

A "Referral from the Doctor" Blog Article- Genomic DNA contamination has been a hot topic for qPCR researchers since ...
Read more

Topics: Real-time PCR

A Quick Jump Start to Multiplexing qPCR

Posted on Wed, Jan 06, 2010 @ 10:01 AM

By now, you've no doubt read about the reductions in error, cost and time that can be achieved by multiplexing ...
Read more

Topics: Real-time PCR

Slope Efficiency Equals Manageable and Corrected PCR

Posted on Wed, Dec 09, 2009 @ 12:01 PM

A "Referral from the Doctor" Blog Article-   ε = m.c.2 (shall I explain? slope efficiency equals manageable and ...
Read more

Topics: Real-time PCR

Bringing Back a Champion: Competitive Quantitative Real-time RT-PCR

Posted on Wed, Nov 18, 2009 @ 08:08 AM

A "Referral from the Doctor" Blog Article- Competitive qRT-PCR has become a lost art. Early titans of probe-based PCR ...
Read more

Topics: Real-time PCR

The New Favorite Reference Gene: 36B4, a.k.a. RPLP0

Posted on Wed, Oct 14, 2009 @ 08:06 AM

A "Referral from the Doctor" Blog Article- Ribosomes are comprised of two subunits, one small (40S) and one large ...
Read more

Topics: Real-time PCR, Oligo synthesis and modifications

What Genes are Hot, What Genes are Not

Posted on Wed, Oct 07, 2009 @ 02:41 PM

 A "Referral from the Doctor" Blog Article-  Top 10 Reference Genes for probe-based Real-time PCR in 2009: • Acidic ...
Read more

Topics: Real-time PCR

Labeling Oligos with Internal BHQ Dyes

Posted on Tue, Sep 29, 2009 @ 08:16 AM

  When to use an internal Black Hole Quencher (BHQ) label in a fluorescence-quenched probe for real-time qPCR. When the ...
Read more

Topics: Real-time PCR, Oligo synthesis and modifications

Salt-Free Primer Pair Option Now Available

Posted on Tue, Sep 22, 2009 @ 08:08 AM

Biosearch Technologies will now be offering the option of salt-free primer pairs for purchase with any probe order. A ...
Read more

Topics: Real-time PCR, Oligo synthesis and modifications

Subscribe to our blog

About LGC, Biosearch Technologies

LGC, Biosearch Technologies is the complete Genomics portfolio from LGC. Providing genomic analysis tools, instrumentation and services to the genomic scientific discovery sector worldwide, with focus on across ag bio, pharma and molecular diagnostics. Visit our home page to view our products and services.

Posts by popularity

Follow @BiosearchTech on Twitter

Become a Fan on Facebook!