It wouldn't be fair to close out 2016 without showcasing the stellar year that Stellaris® RNA FISH has had. Take a closer look at our top 10 highlights from the past year - mind blowing images are only the beginning!Read More
The BiosearchTech Blog
Single Nucleotide Polymorphisms (SNPs) have been widely studied for over 15 years, and have applications in forensics, disease susceptibility studies, pharmacogenomic and other personalized medicine applications, crop and livestock breeding programs. Numerous genetic methods and companion platforms have been developed for SNP detection encompassing DNA sequencing, microarrays, mass spec, and PCR based techniques. For high sample throughput applications, PCR analysis has emerged as the method of choice for rapid targeted SNP analysis, facilitated by new platforms and reagent cost improvements. On October 30, 2014, Biosearch Technologies sponsored a webinar showcasing the utility of the BHQplus® probe technology for qPCR based SNP analysis. Presenter Cassie Keppel, Laboratory Operations Manager at Douglas Scientific, shared some compelling data showing the accurate and economical utility behind the use of Biosearch’s BHQplus probes on Douglas Scientific’s Array Tape® platform. In this article, we highlight some of Cassie’s findings on the dramatic cost-savings that the BHQplus probe and Array Tape platform combination offers.Read More
Don’t waste time performing an extra step. Cut back on pipetting and order a ValuMix™ Assay to have your probes and primers delivered in one tube. The oligos are formulated according to the quantities you specify and delivered dry for maximal stability. ValuMix Assays expedites your research because they:
Biosearch Technologies, Inc. (Biosearch) announced today that the company has implemented a rapid response to the current outbreak of Avian Influenza Virus A (H7N9) in China. Biosearch is ready to provide to the scientific community on-demand, rapid synthesis of real-time qPCR probes and primers composing the H7N9 detection panel recently developed and published on 8 April by the World Health Organization (WHO) Collaborating Center for Reference and Research on Influenza in Beijing, China. WHO lists FAM-BHQ® probes as crucial reagents in their protocol named, “Real-time RT-PCR Protocol for the Detection of A(H7N9) Influenza Virus,” which is available for download at:
Biosearch Technologies today announces that it has entered into a new license relationship with QIAGEN (NASDAQ: QIA, Frankfurt Prime Standard) providing broad commercialisation rights to Scorpions Primers. This agreement allows Biosearch the right to manufacture, catalog, and sell Scorpions primer assays into the research, applied, and infectious disease testing markets.
Biosearch Technologies is pleased to announce that we are offering protein labeling kits using our recently developed water soluble Quasar® WS dyes. These fluorescent dyes have charged substituents that add water solubility and resistance to self-quenching. Quasar 570WS and Quasar 670WS will be provided as succinimidyl active esters for labeling amine groups on proteins and other substrates.
We are pleased to introduce ValuPanel Reagents, a new product line of mission-critical probe and primer sets that discriminate different strains of pathogens. This product line culminated from our successful collaboration with Centers for Disease Control and Prevention* (CDC) in response to the 2009 H1N1 pandemic. As of now, ValuPanel Reagents are available for both 2009 H1N1 and seasonal Influenza A subtyping.
A "Referral from the Doctor" Blog Article-
Scorpions®Primers are dual-labeled FRET probes that combine a Molecular Beacon®-like probe structure and a PCR primer element in a single oligonucleotide, allowing for specific target amplification and advanced target detection through a unimolecular or single oligonucleotide driven mechanism.
Q. What parts are similar to dual-labeled Black Hole Quencher® (BHQ) probes?
A BHQ dye and a 5'-fluorophore covalently bound to the oligonucleotide termini.
When to use an internal Black Hole Quencher (BHQ) label in a fluorescence-quenched probe for real-time qPCR.
When the sequence length of your probe is 30 bases or shorter, we recommend selecting a conventional BHQ label for the 3' end of your probe. We have noticed, however, that probe lengths longer than 30 bases exhibit high background noise and are frequently nonfunctional. They are too long for efficient FRET quenching, a mechanism that is highly dependent upon the distance between the fluorophore and quencher. A shorter average distance between fluorophore and quencher is optimal for a FRET quenching environment, which is common in real-time qPCR experiments.
We have done extensive research to show that the difference in purity specifications does not compromise the quality or performance in assays using the salt-free primer pairs instead of the cartridge purified primer pairs. You can review the Biosearch-conducted purification study by downloading this report.
Since its introduction in 2005, the ValuProbe BHQ Probe has become Biosearch Technologies' most popular product sold to the real-time qPCR industry. Many molecular biologists choose the ValuProbe BHQ Probe because they are quality, superior performing fluorescence-quenched probes that work great for validating and optimizing real-time qPCR assays.