The BiosearchTech Blog

Unraveling the Neural Web: Stellaris® RNA FISH for Neuroscience Research

Posted on Thu, Nov 13, 2014 @ 03:45 PM

Neuroscience gene expression research can come with many hurdles, especially when working with brain tissue or mixed populations of cultured neurons. Here are just a few obstacles Neuroscientists run into:

  1. The brain is not made only of neurons! Alongside neurons, brain tissue can contain a variety of cell types such as glial cells and oligodendrocytes.These cell types may act separately or in conjunction with each other to perform a specific function.
  2. The brain itself is regionally heterogeneous, containing specialized anatomical structures such as the amygdala, hypothalamus, and hippocampus. These regions perform distinct functions such as eliciting the fight or flight response, signaling reproduction, or contributing to memory.
  3. Even so, delving within each of these brain regions, you will find even more specialization. Certain cell populations express a particular RNA signature. For example, tyrosine hydroxylase (Th) expression in dopaminergic neurons, which is critical to the synthesis of the neurotransmitter dopamine. Cells surrounding Th cells, or interspersed with Th cells do not express Th at all! These are all contributing factors that make neuroscience so incredibly fascinating, yet so cumbersome.
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Topics: RNA FISH, Lab Method

Five Ways Controls Can Demystify Your Stellaris® RNA FISH Experiment

Posted on Tue, Nov 04, 2014 @ 02:13 PM

Stellaris controls, who needs ‘em? We all do. Especially if it’s your first time interrogating a sample with a new Stellaris RNA FISH probe set, it is critical to consider the proper controls. The concept of positive and negative controls for experiments is not a new one. Far too often, we tend to bypass the consideration of controls in favor of saving time. However, it really all boils down to, “how do you, as a scientist, interpret your results after the experiment is over?”

Positive and negative controls for Stellaris experiments can be viewed from multiple angles. Let’s say you were successful in measuring spots on your first try, the major questions you may ask are:

  • Is the signal I am measuring being produced by probe bound to RNA?
  • Are these spots true signal or just background?
  • Are these spots specific to my target or are they picking up other RNAs?
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Topics: RNA FISH, Lab Method

Stellaris FISH Workshop at the AMP 2011

Posted on Tue, Nov 15, 2011 @ 01:53 PM

Join us at AMP 2011 on November 16th for a live demonstration of the Stellaris technology at the Gaylord Texan Resort in Grapevine, TX. Biosearch Technologies’ lead Stellaris research scientist, Arturo Orjalo, PhD will demonstrate the preparation and fluorescent imaging of single molecule messenger RNA in fixed cells. The workshop will take place in the Texas 1 room at 1:00PM.

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Topics: RNA FISH, Lab Method

Pipetting for qPCR

Posted on Wed, Jun 09, 2010 @ 08:41 AM

A "Referral from the Doctor" Blog Article-

Research trends in laboratories today increasingly steer towards gene expression analysis and genetic testing, often in the form of qPCR. As reproducibility is essential to genetic research it is imperative that scientists know the fundamentals of micro-volume pipetting.

Forward and Reverse Pipetting: This discussion is limited to the use of manual pipettors. Electronic pipettors are capable of other pipetting techniques such as dispensing, sequential dispensing and diluting which are not discussed here.

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Topics: Real-time PCR, Lab Method, Referral from the Doctor

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About LGC, Biosearch Technologies

LGC, Biosearch Technologies is the complete Genomics portfolio from LGC. Providing genomic analysis tools, instrumentation and services to the genomic scientific discovery sector worldwide, with focus on across ag bio, pharma and molecular diagnostics. Visit our home page to view our products and services.

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