The BiosearchTech Blog

We continue our “Know Your Oligo Mod” series with another versatile custom oligonucleotide modification: the Thio C6 Linker (Thiohexyl) modification.  Figure 1 depicts the reactive handle of a Thio C6 Linker that can be incorporated as either a 5’ modification or a 3’ modification on a custom oligonucleotide. The addition of this custom oligo modification creates a conjugation site with a reactive thiol group.

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This week, we continue our Know Your Oligo Mod series and we will focus on a very basic, yet extremely versatile modification: 3’ Spacer C3. This modification is just a short 3 carbon chain (C3), which is attached to the terminal 3’ hydroxyl group of the oligonucleotide.

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This post in our continuing series, “Know your oligo mod” will cover phosphorothioate bonds, which are available for incorporation into custom oligonucleotides through Biosearch Technologies. This modification is interesting as it is not an addition of a chemical moiety per se, such as a nonstandard base or a quencher, but rather it is a special linkage between the bases. While the canonical DNA backbone found in nature includes phosphodiester linkages between bases, this bond can be altered to a phosphorothioate linkage along the backbone of the oligo (Fig 1).

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The chemical synthesis of oligonucleotides allows for the incorporation of a wealth of modifications to an oligo sequence. However, most scientists usually only consider the addition of fluorescent dyes or quenchers to the terminal ends of their oligo to create the prototypical qPCR probe. There exists a wide range of different modification options allowing for a myriad of diverse applications for synthetic nucleic acid chemistry. This article is the first in the Know Your Oligo Mod blog series which will explore the specialty modifications options which Biosearch Technologies makes available for custom oligo synthesis and to highlight some of their potential applications.

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