The BiosearchTech Blog

Biosearch Technologies has launched qPCRdesign.com, a new microsite targeted specifically for your quantitative PCR needs. In the qPCR Design lab, users can design, analyze, experiment, and play with a variety of interactive tools at their disposal. This is an excellent resource for both new and seasoned qPCR users to access information, advice, or the tools to quickly perform calculations and begin performing assays in no time!

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Topics: Real-time PCR, Design Services

RealTimeDesign (RTD ^TM) Software from Biosearch Technologies is an easy to use, yet powerful assay design application for real-time qPCR. This user-friendly software is available free of charge through our website and requires NO installation. We have recently updated RealTimeDesign software with more options and improvements to the ordering process:

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Topics: Real-time PCR, Design Services

By now, you've no doubt read about the reductions in error, cost and time that can be achieved by multiplexing real time PCR. A common complaint about multiplexing is that, despite its benefits, it is sometimes considered too much of a hassle. This post will address some key factors to consider when planning a multiplexed qPCR assay.

1. Instrumentation and Dye Selection
This one is pretty easy. For a successful multiplex, your instrument needs to be able to excite each of your reporter dyes. Although there are dyes on the market that excite in the far red, you'll be safe using an instrument which excites over the visible spectrum (350-750nm). Biosearch Technologies has developed two essential tools to assist you with dye selection:

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Topics: Real-time PCR, Design Services