The BiosearchTech Blog

Labeling Oligos with Internal BHQ Dyes

Posted on Tue, Sep 29, 2009 @ 08:16 AM

 

When to use an internal Black Hole Quencher (BHQ) label in a fluorescence-quenched probe for real-time qPCR.

When the sequence length of your probe is 30 bases or shorter, we recommend selecting a conventional BHQ label for the 3' end of your probe. We have noticed, however, that probe lengths longer than 30 bases exhibit high background noise and are frequently nonfunctional. They are too long for efficient FRET quenching, a mechanism that is highly dependent upon the distance between the fluorophore and quencher. A shorter average distance between fluorophore and quencher is optimal for a FRET quenching environment, which is common in real-time qPCR experiments.

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Topics: Real-time PCR, Product Information, Custom Oligos

Salt-Free Primer Pair Option Now Available

Posted on Tue, Sep 22, 2009 @ 08:08 AM

Biosearch Technologies will now be offering the option of salt-free primer pairs for purchase with any probe order. A more affordable option for real-time qPCR, these primers offer a higher yield at a faster turnaround time in comparison to cartridge-purified primers.

We have done extensive research to show that the difference in purity specifications does not compromise the quality or performance in assays using the salt-free primer pairs instead of the cartridge purified primer pairs. You can review the Biosearch-conducted purification study by downloading this report.

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Topics: Real-time PCR, Product Information, Custom Oligos

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About Biosearch Technologies

As the inventor of the BHQ dyes, Biosearch Technologies synthesizes sophisticated oligos for real time qPCR, molecular diagnostics, and more!  Visit our home page to view our products and services.

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