One Step too Many? Simplify your qPCR Protocol.

Posted on Thu, Feb 27, 2014 @ 10:02 AM

Don’t waste time performing an extra step. Cut back on pipetting and order a ValuMix™ Assay to have your probes and primers delivered in one tube. The oligos are formulated according to the quantities you specify and delivered dry for maximal stability. ValuMix Assays expedites your research because they:

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  • Reduce waste by delivering defined probe and primer amounts
  • Minimize pipetting errors with fewer liquid handling steps
  • Simplify and accelerate your protocol

Read on to learn more.

ValuMix Assays for Gene Expression and qPCR

For qPCR applications such as gene expression analysis, a ValuMix Assay contains one BHQ® probe mixed with two standard PCR primers. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. With delivered amounts of probe starting from 0.5 nmol, ValuMix Assays for gene expression is ideal for high-throughput, nanoliter thermal cyclers such as with digital or array-based qPCR. Fluorophore options include FAM, CAL Fluor® Orange 560, CAL Fluor Red 610 or Quasar® 670 dyes. Please refer to our dye selection chart for more information.

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View our poster for additional details
Genotyping with BHQplus probes

ValuMix Assays for SNP Genotyping

If your work requires probe-based SNP genotyping, we can provide two BHQplus® probes and primers mixed into a single tube. BHQplus probes incorporate a duplex-stabilizing chemistry that permits the design of shorter oligonucleotide probes while maintaining the elevated melting temperature (Tm) that is optimal for real-time PCR. These probes are formulated for improved specificity, stronger hybridization, and a smaller binding footprint, making them ideal for the analysis of single nucleotide polymorphisms or species discrimination. For more information please refer to the SNP genotyping applications page or view the real-time PCR probe animation.

Whether marker-assisted selection, or screening for rare alleles that influence health, consider ValuMix SNP Assays to accelerate your genotyping research.

Topics: Real-time PCR, Product Information

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About Biosearch Technologies

As the inventor of the BHQ dyes, Biosearch Technologies synthesizes sophisticated oligos for real time qPCR, molecular diagnostics, and more!  Visit our home page to view our products and services.

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